Isolation of C-phycocyanin from Spirulina platensis microalga using Ionic liquid based aqueous two-phase system

Yu Kaung Chang, Pau Loke Show, John Chi Wei Lan, Jung Chin Tsai, Chi Rong Huang

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

An aqueous two-phase system (ATPS) with ionic liquids (ILs) was used for the isolate of C-phycocyanin (CPC) from Spirulina platensis microalga. Various imidazolium ILs and potassium salts were studied. The effect of ILs-ATPS on the extraction efficiency of CPC was also studied. The experimental parameters like pH, loading volume, algae concentration, temperature, and alkyl chain length of IL were well-covered in this report. The experimental results showed that the extraction efficiency, the partition coefficient, and the separation factor for CPC were 99%, 36.6, and 5.8, respectively, for an optimal pH value of 7 and a temperature of 308 K. The order of extraction efficiency for CPC using IL-ATPS was: 1-octyl-3-methylimidazolium bromide (C8MIM-Br) > 1-hexyl-3-methylimidazolium bromide (C6MIM-Br) > 1-butyl-3-methylimidazolium bromide (C4MIM-Br). The isolation process followed the pseudo second-order kinetic model and the thermodynamic results were obviously spontaneous.

Original languageBritish English
Pages (from-to)320-327
Number of pages8
JournalBioresource Technology
Volume270
DOIs
StatePublished - Dec 2018

Keywords

  • Aqueous two-phase system
  • C-phycocyanin
  • Ionic liquids
  • Isolation
  • Spirulina platensis

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