Isolation and antioxidant activity of flavonoids from Holarrhena floribunda (G.don) leaves

Jelili A. Badmus; Okobi E. Ekpo; Fanie Rautenbach; Jeanine L. Marnewick; Ahmed A. Hussein; Donavon C. Hiss

doi:10.18388/abp.2015_1178

Isolation and antioxidant activity of flavonoids from Holarrhena floribunda (G.don) leaves

Jelili A. Badmus, Okobi E. Ekpo, Fanie Rautenbach, Jeanine L. Marnewick, Ahmed A. Hussein, Donavon C. Hiss

College of Medicine and Health Sciences

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Bioactive polyphenolics are ubiquitously present in plants and may play an important role in the prevention and management of certain human diseases. Three known flavonoids viz Kaemperol-3-O-rutinoside (1), quercetin-3-O-glucoside (2) and kaemperol-3-O-glucoside (3) and inseparable mixture (1:1) of quercetin-3-Oglucose/galactose (4) were isolated, and identified for the first time from Holarrhena floribunda. The antioxidant capacity using the ORAC, FRAP and TEAC assays and inhibition of lipid peroxidation were measured for isolated flavonoids. The result showed that compounds 2 and 4 showed significantly increased ORAC, TEAC, and FRAP activities with low pro-oxidant potential as well as improved lipid peroxidation inhibition levels when compared to compounds 1 and 3. The most active compounds were found to be flavonoids with a quercetin basic structure. These results imply that the isolated flavonoid glycosides are responsible for the antioxidant activity of the plant leaves and it forms the scientific basis for its traditional usage.

Original language	British English
Pages (from-to)	353-358
Number of pages	6
Journal	Acta Biochimica Polonica
Volume	63
Issue number	2
DOIs	https://doi.org/10.18388/abp.2015_1178
State	Published - 2016

Keywords

Antioxidant
Flavonoids
Holarrhena floribunda
Plants
Polyphenolics

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@article{d92018711cd141578f9a912bf10e8376,

title = "Isolation and antioxidant activity of flavonoids from Holarrhena floribunda (G.don) leaves",

abstract = "Bioactive polyphenolics are ubiquitously present in plants and may play an important role in the prevention and management of certain human diseases. Three known flavonoids viz Kaemperol-3-O-rutinoside (1), quercetin-3-O-glucoside (2) and kaemperol-3-O-glucoside (3) and inseparable mixture (1:1) of quercetin-3-Oglucose/galactose (4) were isolated, and identified for the first time from Holarrhena floribunda. The antioxidant capacity using the ORAC, FRAP and TEAC assays and inhibition of lipid peroxidation were measured for isolated flavonoids. The result showed that compounds 2 and 4 showed significantly increased ORAC, TEAC, and FRAP activities with low pro-oxidant potential as well as improved lipid peroxidation inhibition levels when compared to compounds 1 and 3. The most active compounds were found to be flavonoids with a quercetin basic structure. These results imply that the isolated flavonoid glycosides are responsible for the antioxidant activity of the plant leaves and it forms the scientific basis for its traditional usage.",

keywords = "Antioxidant, Flavonoids, Holarrhena floribunda, Plants, Polyphenolics",

author = "Badmus, {Jelili A.} and Ekpo, {Okobi E.} and Fanie Rautenbach and Marnewick, {Jeanine L.} and Hussein, {Ahmed A.} and Hiss, {Donavon C.}",

note = "Funding Information: The authors thank TETFund (Nigeria) for PhD travel scholarship granted to the first author. The work was sponsored, in part, by the National Research Foundation (NRF), South Africa.",

year = "2016",

doi = "10.18388/abp.2015_1178",

language = "British English",

volume = "63",

pages = "353--358",

journal = "Acta Biochimica Polonica",

issn = "0001-527X",

publisher = "Frontiers Media SA",

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TY - JOUR

T1 - Isolation and antioxidant activity of flavonoids from Holarrhena floribunda (G.don) leaves

AU - Badmus, Jelili A.

AU - Ekpo, Okobi E.

AU - Rautenbach, Fanie

AU - Marnewick, Jeanine L.

AU - Hussein, Ahmed A.

AU - Hiss, Donavon C.

N1 - Funding Information: The authors thank TETFund (Nigeria) for PhD travel scholarship granted to the first author. The work was sponsored, in part, by the National Research Foundation (NRF), South Africa.

PY - 2016

Y1 - 2016

N2 - Bioactive polyphenolics are ubiquitously present in plants and may play an important role in the prevention and management of certain human diseases. Three known flavonoids viz Kaemperol-3-O-rutinoside (1), quercetin-3-O-glucoside (2) and kaemperol-3-O-glucoside (3) and inseparable mixture (1:1) of quercetin-3-Oglucose/galactose (4) were isolated, and identified for the first time from Holarrhena floribunda. The antioxidant capacity using the ORAC, FRAP and TEAC assays and inhibition of lipid peroxidation were measured for isolated flavonoids. The result showed that compounds 2 and 4 showed significantly increased ORAC, TEAC, and FRAP activities with low pro-oxidant potential as well as improved lipid peroxidation inhibition levels when compared to compounds 1 and 3. The most active compounds were found to be flavonoids with a quercetin basic structure. These results imply that the isolated flavonoid glycosides are responsible for the antioxidant activity of the plant leaves and it forms the scientific basis for its traditional usage.

AB - Bioactive polyphenolics are ubiquitously present in plants and may play an important role in the prevention and management of certain human diseases. Three known flavonoids viz Kaemperol-3-O-rutinoside (1), quercetin-3-O-glucoside (2) and kaemperol-3-O-glucoside (3) and inseparable mixture (1:1) of quercetin-3-Oglucose/galactose (4) were isolated, and identified for the first time from Holarrhena floribunda. The antioxidant capacity using the ORAC, FRAP and TEAC assays and inhibition of lipid peroxidation were measured for isolated flavonoids. The result showed that compounds 2 and 4 showed significantly increased ORAC, TEAC, and FRAP activities with low pro-oxidant potential as well as improved lipid peroxidation inhibition levels when compared to compounds 1 and 3. The most active compounds were found to be flavonoids with a quercetin basic structure. These results imply that the isolated flavonoid glycosides are responsible for the antioxidant activity of the plant leaves and it forms the scientific basis for its traditional usage.

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KW - Holarrhena floribunda

KW - Plants

KW - Polyphenolics

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ER -

Isolation and antioxidant activity of flavonoids from Holarrhena floribunda (G.don) leaves

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Keywords

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