Integrated ultrasound-assisted liquid biphasic flotation for efficient extraction of astaxanthin from Haematococcus pluvialis

Kuan Shiong Khoo; Kit Wayne Chew; Guo Yong Yew; Sivakumar Manickam; Chien Wei Ooi; Pau Loke Show

doi:10.1016/j.ultsonch.2020.105052

Integrated ultrasound-assisted liquid biphasic flotation for efficient extraction of astaxanthin from Haematococcus pluvialis

Kuan Shiong Khoo, Kit Wayne Chew, Guo Yong Yew, Sivakumar Manickam, Chien Wei Ooi, Pau Loke Show

Chemical and Petroleum Engineering

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

The purpose of this investigation is to evaluate the implementation of ultrasound-assisted liquid biphasic flotation (LBF) system for the recovery of natural astaxanthin from Haematococcus pluvialis microalgae. Various operating conditions of ultrasound-assisted LBF systems such as the position of ultrasound horn, mode of ultrasonication (pulse and continuous), amplitude of ultrasonication, air flowrate, duration of air flotation, and mass of H. pluvialis microalgae were evaluated. The effect of ultrasonication on the cellular morphology of microalgae was also assessed using microscopic analysis. Under the optimized operating conditions of UALBF, the maximum recovery yield, extraction efficiency, and partition coefficient of astaxanthin were 95.08 ± 3.02%, 99.74 ± 0.05%, and 185.09 ± 4.78, respectively. In addition, the successful scale-up operation of ultrasound-assisted LBF system verified the practicability of this integrated approach for an effective extraction of natural astaxanthin.

Original language	British English
Article number	105052
Journal	Ultrasonics Sonochemistry
Volume	67
DOIs	https://doi.org/10.1016/j.ultsonch.2020.105052
State	Published - Oct 2020

Keywords

Astaxanthin
Cavitation
Extraction
Haematococcus pluvialis
Liquid biphasic flotation
Ultrasound

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10.1016/j.ultsonch.2020.105052

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title = "Integrated ultrasound-assisted liquid biphasic flotation for efficient extraction of astaxanthin from Haematococcus pluvialis",

abstract = "The purpose of this investigation is to evaluate the implementation of ultrasound-assisted liquid biphasic flotation (LBF) system for the recovery of natural astaxanthin from Haematococcus pluvialis microalgae. Various operating conditions of ultrasound-assisted LBF systems such as the position of ultrasound horn, mode of ultrasonication (pulse and continuous), amplitude of ultrasonication, air flowrate, duration of air flotation, and mass of H. pluvialis microalgae were evaluated. The effect of ultrasonication on the cellular morphology of microalgae was also assessed using microscopic analysis. Under the optimized operating conditions of UALBF, the maximum recovery yield, extraction efficiency, and partition coefficient of astaxanthin were 95.08 ± 3.02%, 99.74 ± 0.05%, and 185.09 ± 4.78, respectively. In addition, the successful scale-up operation of ultrasound-assisted LBF system verified the practicability of this integrated approach for an effective extraction of natural astaxanthin.",

keywords = "Astaxanthin, Cavitation, Extraction, Haematococcus pluvialis, Liquid biphasic flotation, Ultrasound",

author = "Khoo, {Kuan Shiong} and Chew, {Kit Wayne} and Yew, {Guo Yong} and Sivakumar Manickam and Ooi, {Chien Wei} and Show, {Pau Loke}",

note = "Funding Information: This work was supported by the Fundamental Research Grant Scheme, Malaysia [ FRGS/1/2019/STG05/UNIM/02/2 ], and Monash University Malaysia Sustainable Community Grant Scheme [ SDG-2018-04-SCI ]. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = oct,

doi = "10.1016/j.ultsonch.2020.105052",

language = "British English",

volume = "67",

journal = "Ultrasonics Sonochemistry",

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AU - Khoo, Kuan Shiong

AU - Chew, Kit Wayne

AU - Yew, Guo Yong

AU - Manickam, Sivakumar

AU - Ooi, Chien Wei

AU - Show, Pau Loke

N1 - Funding Information: This work was supported by the Fundamental Research Grant Scheme, Malaysia [ FRGS/1/2019/STG05/UNIM/02/2 ], and Monash University Malaysia Sustainable Community Grant Scheme [ SDG-2018-04-SCI ]. Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/10

Y1 - 2020/10

N2 - The purpose of this investigation is to evaluate the implementation of ultrasound-assisted liquid biphasic flotation (LBF) system for the recovery of natural astaxanthin from Haematococcus pluvialis microalgae. Various operating conditions of ultrasound-assisted LBF systems such as the position of ultrasound horn, mode of ultrasonication (pulse and continuous), amplitude of ultrasonication, air flowrate, duration of air flotation, and mass of H. pluvialis microalgae were evaluated. The effect of ultrasonication on the cellular morphology of microalgae was also assessed using microscopic analysis. Under the optimized operating conditions of UALBF, the maximum recovery yield, extraction efficiency, and partition coefficient of astaxanthin were 95.08 ± 3.02%, 99.74 ± 0.05%, and 185.09 ± 4.78, respectively. In addition, the successful scale-up operation of ultrasound-assisted LBF system verified the practicability of this integrated approach for an effective extraction of natural astaxanthin.

AB - The purpose of this investigation is to evaluate the implementation of ultrasound-assisted liquid biphasic flotation (LBF) system for the recovery of natural astaxanthin from Haematococcus pluvialis microalgae. Various operating conditions of ultrasound-assisted LBF systems such as the position of ultrasound horn, mode of ultrasonication (pulse and continuous), amplitude of ultrasonication, air flowrate, duration of air flotation, and mass of H. pluvialis microalgae were evaluated. The effect of ultrasonication on the cellular morphology of microalgae was also assessed using microscopic analysis. Under the optimized operating conditions of UALBF, the maximum recovery yield, extraction efficiency, and partition coefficient of astaxanthin were 95.08 ± 3.02%, 99.74 ± 0.05%, and 185.09 ± 4.78, respectively. In addition, the successful scale-up operation of ultrasound-assisted LBF system verified the practicability of this integrated approach for an effective extraction of natural astaxanthin.

KW - Astaxanthin

KW - Cavitation

KW - Extraction

KW - Haematococcus pluvialis

KW - Liquid biphasic flotation

KW - Ultrasound

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SN - 1350-4177

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Integrated ultrasound-assisted liquid biphasic flotation for efficient extraction of astaxanthin from Haematococcus pluvialis

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