Immobilization of angiotensin II and bovine serum albumin on strip-patterned ZnO nanorod arrays

Q. Ahsanulhaq, J. H. Kim, Y. B. Hahn

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

For an effective protein immobilization for highly sensitive biosensors, we determined the binding properties and characteristics of angiotensin II and bovine serum albumin on the surface of patterned ZnO nanorod arrays (NRAs) which were selectively grown on desired areas of Si substrates. The surfaces of ZnO NRAs were modified by 3-aminopropyltriethoxysilane and gluteraldehyde, and the activated NRAs were then conjugated with angiotensin II protein and bovine serum albumin. The silanization process and conjugation of protein were verified by secondary ion mass spectroscopy (SIMS) and X-ray photoelectron spectroscopy (XPS) techniques. The immobilizing densities of proteins determined by Coomassie protein assay were 4.5 JxgJcm2 for angiotensin II and 5.3 μg/cm 2 for bovine serum albumin.

Original languageBritish English
Pages (from-to)4159-4165
Number of pages7
JournalJournal of Nanoscience and Nanotechnology
Volume10
Issue number7
DOIs
StatePublished - Jul 2010

Keywords

  • Angiotensin II
  • Bovine Serum Albumin
  • Immobilization
  • ZnO Nanorod Arrays

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