HPLC determination of K027 in the body of pregnant mice

S. M. Nurulain; S. Ojha; S. Dhanasekaran; K. Kuča; N. Nalin; C. Sharma; A. Adem; H. Kalász

doi:10.1556/1326.2017.29.1.06

HPLC determination of K027 in the body of pregnant mice

S. M. Nurulain, S. Ojha, S. Dhanasekaran, K. Kuča, N. Nalin, C. Sharma, A. Adem, H. Kalász

College of Medicine and Health Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Distribution of K027, a hydrophilic, positively charged compound is monitored in the body of pregnant mice using high-performance liquid chromatography (HPLC). Intraperitoneal injection was done on the 18th day of pregnancy; the plasma and brains of the mother mice, placentae and the fetuses' brains were dissected following 5, 15, 30, 60, and 120 min of treatment. Significant incorporation of K027 was found in the placentae and in fetuses' brains relative to its levels in the mothers' plasma and brains. This incorporation warns of a possible adjustment of dose of pyridinium aldoxime antidotes in case of pregnancy. Further studies with different gestational periods and animal models are warranted.

Original language	British English
Pages (from-to)	85-93
Number of pages	9
Journal	Acta Chromatographica
Volume	29
Issue number	1
DOIs	https://doi.org/10.1556/1326.2017.29.1.06
State	Published - Mar 2017

Keywords

Blood-placenta distribution
Drug distribution
HPLC
K027
Pregnant mice
Pups

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10.1556/1326.2017.29.1.06

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title = "HPLC determination of K027 in the body of pregnant mice",

abstract = "Distribution of K027, a hydrophilic, positively charged compound is monitored in the body of pregnant mice using high-performance liquid chromatography (HPLC). Intraperitoneal injection was done on the 18th day of pregnancy; the plasma and brains of the mother mice, placentae and the fetuses' brains were dissected following 5, 15, 30, 60, and 120 min of treatment. Significant incorporation of K027 was found in the placentae and in fetuses' brains relative to its levels in the mothers' plasma and brains. This incorporation warns of a possible adjustment of dose of pyridinium aldoxime antidotes in case of pregnancy. Further studies with different gestational periods and animal models are warranted.",

keywords = "Blood-placenta distribution, Drug distribution, HPLC, K027, Pregnant mice, Pups",

author = "Nurulain, {S. M.} and S. Ojha and S. Dhanasekaran and K. Ku{\v c}a and N. Nalin and C. Sharma and A. Adem and H. Kal{\'a}sz",

note = "Funding Information: Thanks are due to Mr. M. Shafiullah and Mr. J. Horv{\'a}th for their advice. Dr. Kal{\'a}sz contribution was sponsored by OTKA 100155 grant of Hungarian National Scientific Granting Agency (Budapest, Hungary), and Dr. Ojha's contribution was supported in part by grants from United Arab Emirates University and United Arab Emirates National Research Foundation. Publisher Copyright: {\textcopyright} 2016 The Author(s).",

year = "2017",

month = mar,

doi = "10.1556/1326.2017.29.1.06",

language = "British English",

volume = "29",

pages = "85--93",

journal = "Acta Chromatographica",

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AU - Nurulain, S. M.

AU - Ojha, S.

AU - Dhanasekaran, S.

AU - Kuča, K.

AU - Nalin, N.

AU - Sharma, C.

AU - Adem, A.

AU - Kalász, H.

N1 - Funding Information: Thanks are due to Mr. M. Shafiullah and Mr. J. Horváth for their advice. Dr. Kalász contribution was sponsored by OTKA 100155 grant of Hungarian National Scientific Granting Agency (Budapest, Hungary), and Dr. Ojha's contribution was supported in part by grants from United Arab Emirates University and United Arab Emirates National Research Foundation. Publisher Copyright: © 2016 The Author(s).

PY - 2017/3

Y1 - 2017/3

N2 - Distribution of K027, a hydrophilic, positively charged compound is monitored in the body of pregnant mice using high-performance liquid chromatography (HPLC). Intraperitoneal injection was done on the 18th day of pregnancy; the plasma and brains of the mother mice, placentae and the fetuses' brains were dissected following 5, 15, 30, 60, and 120 min of treatment. Significant incorporation of K027 was found in the placentae and in fetuses' brains relative to its levels in the mothers' plasma and brains. This incorporation warns of a possible adjustment of dose of pyridinium aldoxime antidotes in case of pregnancy. Further studies with different gestational periods and animal models are warranted.

AB - Distribution of K027, a hydrophilic, positively charged compound is monitored in the body of pregnant mice using high-performance liquid chromatography (HPLC). Intraperitoneal injection was done on the 18th day of pregnancy; the plasma and brains of the mother mice, placentae and the fetuses' brains were dissected following 5, 15, 30, 60, and 120 min of treatment. Significant incorporation of K027 was found in the placentae and in fetuses' brains relative to its levels in the mothers' plasma and brains. This incorporation warns of a possible adjustment of dose of pyridinium aldoxime antidotes in case of pregnancy. Further studies with different gestational periods and animal models are warranted.

KW - Blood-placenta distribution

KW - Drug distribution

KW - HPLC

KW - K027

KW - Pregnant mice

KW - Pups

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DO - 10.1556/1326.2017.29.1.06

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SN - 1233-2356

VL - 29

SP - 85

EP - 93

JO - Acta Chromatographica

JF - Acta Chromatographica

IS - 1

ER -

HPLC determination of K027 in the body of pregnant mice

Abstract

Keywords

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