Hoechst-IR: An imaging agent that detects necrotic tissue in vivo by binding extracellular DNA

Madhuri Dasari; Sungmun Lee; Jay Sy; Dongin Kim; Seungjun Lee; Milton Brown; Michael Davis; Niren Murthy

doi:10.1021/ol100923d

Hoechst-IR: An imaging agent that detects necrotic tissue in vivo by binding extracellular DNA

Madhuri Dasari
, Sungmun Lee
, Jay Sy
, Dongin Kim
, Seungjun Lee
, Milton Brown
, Michael Davis
, Niren Murthy

Biomedical Engineering & Biotechnology

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

(Equation Presented). Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.

Original language	British English
Pages (from-to)	3300-3303
Number of pages	4
Journal	Organic Letters
Volume	12
Issue number	15
DOIs	https://doi.org/10.1021/ol100923d
State	Published - 6 Aug 2010

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10.1021/ol100923d

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title = "Hoechst-IR: An imaging agent that detects necrotic tissue in vivo by binding extracellular DNA",

abstract = "(Equation Presented). Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.",

author = "Madhuri Dasari and Sungmun Lee and Jay Sy and Dongin Kim and Seungjun Lee and Milton Brown and Michael Davis and Niren Murthy",

year = "2010",

month = aug,

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doi = "10.1021/ol100923d",

language = "British English",

volume = "12",

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T1 - Hoechst-IR

T2 - An imaging agent that detects necrotic tissue in vivo by binding extracellular DNA

AU - Dasari, Madhuri

AU - Lee, Sungmun

AU - Sy, Jay

AU - Kim, Dongin

AU - Lee, Seungjun

AU - Brown, Milton

AU - Davis, Michael

AU - Murthy, Niren

PY - 2010/8/6

Y1 - 2010/8/6

N2 - (Equation Presented). Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.

AB - (Equation Presented). Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.

UR - https://www.scopus.com/pages/publications/77955133412

U2 - 10.1021/ol100923d

DO - 10.1021/ol100923d

M3 - Article

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AN - SCOPUS:77955133412

SN - 1523-7060

VL - 12

SP - 3300

EP - 3303

JO - Organic Letters

JF - Organic Letters

IS - 15

ER -

Hoechst-IR: An imaging agent that detects necrotic tissue in vivo by binding extracellular DNA

Abstract

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