Highly sensitive and selective hydrogen peroxide biosensor based on hemoglobin immobilized at multiwalled carbon nanotubes-zinc oxide composite electrode

Selvakumar Palanisamy, Srikanth Cheemalapati, Shen Ming Chen

    Research output: Contribution to journalArticlepeer-review

    69 Scopus citations

    Abstract

    A highly sensitive and selective amperometric hydrogen peroxide (H 2O2) biosensor based on immobilization of hemoglobin (Hb) at multiwalled carbon nanotubes-zinc oxide (MWCNT/ZnO) composite modified glassy carbon electrode (GCE) is reported. ZnO microsponges were electrochemically grown on MWCNT surface by the simple, cost-effective, green, electrochemical method at room temperature. The MWCNT/ZnO/Hb composite film showed a pair of well-defined, quasi-reversible redox peaks with a formal potential (E°′) of -0.336 V, characteristic features of heme redox couple of Hb. The electron transfer rate constant (ks) of immobilized Hb was 1.26 s-1. The developed biosensor showed a very fast response (>2 s) toward H2O2 with good sensitivity, wide linear range, and low detection limit of 0.02 μM. The fabricated biosensor showed interesting features, including high selectivity, acceptable stability, good reproducibility, and repeatability along with excellent conductivity, facile electron mobility of MWCNT, and good biocompatibility of ZnO. The fabrication method of this biosensor is simple and effective for determination of H 2O2 in real samples with quick response, good sensitivity, high selectivity, and acceptable recovery.

    Original languageBritish English
    Pages (from-to)108-115
    Number of pages8
    JournalAnalytical Biochemistry
    Volume429
    Issue number2
    DOIs
    StatePublished - 15 Oct 2012

    Keywords

    • Direct electrochemistry
    • Electrodeposition
    • Hemoglobin
    • MWCNT/ZnO

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