Heterodyne detected transient grating UV/VIS-pump IR-probe measurements of energy transport through proteins

Halina Strzalka, Shabir Hassan, Paul M. Donaldson, Peter Hamm

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The transient grating technique is applied to UV/VIS-pump IR-probe measurements to obtain a background free transient infrared signal. Two infrared probe beams, which are diffracted off a population grating in the sample, are heterodyne detected. The setup is passively phase stabilized. In comparison with conventional UV/VIS-pump IR-probe measurements the signal to noise is enhanced by a factor of 25. This enables us to follow IR transient absorption changes of single site labels in proteins in the sub-mM concentration range. As a first application we study the energy transport along proteins.

Original languageBritish English
Title of host publicationInternational Conference on Ultrafast Structural Dynamics, ICUSD 2012
DOIs
StatePublished - 2012
EventInternational Conference on Ultrafast Structural Dynamics, ICUSD 2012 - Berlin, Germany
Duration: 19 Mar 201221 Mar 2012

Publication series

NameInternational Conference on Ultrafast Structural Dynamics, ICUSD 2012

Conference

ConferenceInternational Conference on Ultrafast Structural Dynamics, ICUSD 2012
Country/TerritoryGermany
CityBerlin
Period19/03/1221/03/12

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