Green biorefinery: Microalgae-bacteria microbiome on tolerance investigations in plants

Dong Bui-Xuan, Doris Ying Ying Tang, Kit Wayne Chew, Thi Dong Phuong Nguyen, Han Le Ho, Thi Ngoc Thu Tran, Toan Nguyen-Sy, Thi Ha Thuong Dinh, Phuc Son Nguyen, Thi My Huong Dinh, Thanh Thuy Nguyen, Pau Loke Show

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations


Co-culture of microalgae and microorganisms, supported with the resulting synergistic effects, can be used for wastewater treatment, biomass production, agricultural applications and etc. Therefore, this study aimed to explore the role of Bacillus subtilis (B. subtilis) in tolerance against the harsh environment of seafood wastewater, at which these microalgal-bacterial flocs were formed by microalgae cultivation. In this present study, B. subtilis isolated from the cultivation medium of Chlorella vulgaris and exposed to different salinity (0.1–4% w/v sodium chloride) and various pH range to determine the tolerant ability and biofilm formation. Interestingly, this bacteria strain that isolated from microalgae cultivation medium showed the intense viability in the salt concentration exceeding up to 4% (w/v) NaCl but demonstrated the decrease in cell division as environmental culture undergoing over pH 10. Cell viability was recorded higher than 71% and 92% for B. subtilis inoculum in media with salt concentration greater than 20 gL−1 and external pH 6.5–9, respectively. This showed that B. subtilis isolated from microalgal-bacteria cocultivation exhibited its tolerant ability to survive in the extremely harsh conditions and thus, mitigating the stresses due to salinity and pH.

Original languageBritish English
Pages (from-to)120-127
Number of pages8
JournalJournal of Biotechnology
StatePublished - 10 Jan 2022


  • Bacillus sp.
  • Biofilm
  • Microalgae
  • PH tolerance
  • Salinity tolerance


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