@article{f5a2ee60ef334cd38f7a6db69f16e66c,
title = "Generation of the UAE's first Emirati induced pluripotent stem cell line KUSTi001-A from peripheral blood derived CD34+ hematopoietic cells",
abstract = "Here we report the generation of the first Emirati iPSC line in the United Arab Emirates and name it KUSTi001-A. CD34+ hematopoietic cells purified from peripheral blood of a 27-year-old healthy female donor were reprogrammed using Sendai vectors. Twenty days post-reprogramming colonies were manually picked, and expanded clones were verified for transgene clearance by RT-PCR. Pluripotency was validated by pluripotency genes and differentiation into all three germ layers. Finally, chromosome stability was confirmed by testing 8 common abnormality loci. KUSTi001-A, alternatively called UAE001, is an Emirati hiPSC line that holds great potential for UAE specific regenerative medicine, disease modelling and drug screening.",
author = "Azzam, {Sarah Kassem} and Habiba Alsafar and Sajini, {Abdulrahim A.}",
note = "Funding Information: We would like to thank Dr. James Dutton from the University of Minnesota for sharing his experiences on hiPSC generation and both Dr. Sarah Chehade and Mr. Hussein Kannout from Khalifa University Centre for Biotechnology, for providing training on the Genetic Analyzer 3500 to run STR profiling experiments. This research was funded by Khalifa University of Science and Technology Competitive Internal Research Award CIRA-ADEK and by Abu Dhabi Award for Research Excellence (AARE) 2019. Funding Information: We would like to thank Dr. James Dutton from the University of Minnesota for sharing his experiences on hiPSC generation and both Dr. Sarah Chehade and Mr. Hussein Kannout from Khalifa University Centre for Biotechnology, for providing training on the Genetic Analyzer 3500 to run STR profiling experiments. This research was funded by Khalifa University of Science and Technology Competitive Internal Research Award CIRA-ADEK and by Abu Dhabi Award for Research Excellence (AARE) 2019. Publisher Copyright: {\textcopyright} 2022",
year = "2022",
month = aug,
doi = "10.1016/j.scr.2022.102853",
language = "British English",
volume = "63",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
}