Fundamental studies of DNA adsorption and hybridization on solid surfaces

David J. Graves; Lynn Anne Sanguedolce; Vincent Chan; Steven E. McKenzie; Saul Surrey; Paolo Fortina

Fundamental studies of DNA adsorption and hybridization on solid surfaces

David J. Graves
, Lynn Anne Sanguedolce
, Vincent Chan
, Steven E. McKenzie
, Saul Surrey
, Paolo Fortina

Biomedical Engineering & Biotechnology

University of Pennsylvania

Research output: Contribution to journal › Conference article › peer-review

Abstract

Results are presented of fundamental studies of DNA adsorption and hybridization on solid surfaces. Fluorescence recovery after photobleaching (FRAP) is used to study the intrinsic desorption kinetics for oligonucleotides adsorbed on silanized glass. The results suggest, that are in agreement with the work of others, that the nucleotide is lying down and interacting rather strongly with the treated glass. Addition of alcohol tended to decrease adsorption on the uncharged surfaces and to increase it on the charged amino-propyl surface.

Original language	British English
Pages (from-to)	546
Number of pages	1
Journal	Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Volume	76
State	Published - 1997
Event	Proceedings of the 1997 Spring ACS Meeting - San Francisco, CA, USA Duration: 13 Apr 1997 → 17 Apr 1997

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title = "Fundamental studies of DNA adsorption and hybridization on solid surfaces",

abstract = "Results are presented of fundamental studies of DNA adsorption and hybridization on solid surfaces. Fluorescence recovery after photobleaching (FRAP) is used to study the intrinsic desorption kinetics for oligonucleotides adsorbed on silanized glass. The results suggest, that are in agreement with the work of others, that the nucleotide is lying down and interacting rather strongly with the treated glass. Addition of alcohol tended to decrease adsorption on the uncharged surfaces and to increase it on the charged amino-propyl surface.",

author = "Graves, \{David J.\} and Sanguedolce, \{Lynn Anne\} and Vincent Chan and McKenzie, \{Steven E.\} and Saul Surrey and Paolo Fortina",

year = "1997",

language = "British English",

volume = "76",

pages = "546",

journal = "Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering",

issn = "0743-0515",

note = "Proceedings of the 1997 Spring ACS Meeting ; Conference date: 13-04-1997 Through 17-04-1997",

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Fundamental studies of DNA adsorption and hybridization on solid surfaces. / Graves, David J.; Sanguedolce, Lynn Anne; Chan, Vincent et al.
In: Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering, Vol. 76, 1997, p. 546.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Fundamental studies of DNA adsorption and hybridization on solid surfaces

AU - Graves, David J.

AU - Sanguedolce, Lynn Anne

AU - Chan, Vincent

AU - McKenzie, Steven E.

AU - Surrey, Saul

AU - Fortina, Paolo

PY - 1997

Y1 - 1997

N2 - Results are presented of fundamental studies of DNA adsorption and hybridization on solid surfaces. Fluorescence recovery after photobleaching (FRAP) is used to study the intrinsic desorption kinetics for oligonucleotides adsorbed on silanized glass. The results suggest, that are in agreement with the work of others, that the nucleotide is lying down and interacting rather strongly with the treated glass. Addition of alcohol tended to decrease adsorption on the uncharged surfaces and to increase it on the charged amino-propyl surface.

AB - Results are presented of fundamental studies of DNA adsorption and hybridization on solid surfaces. Fluorescence recovery after photobleaching (FRAP) is used to study the intrinsic desorption kinetics for oligonucleotides adsorbed on silanized glass. The results suggest, that are in agreement with the work of others, that the nucleotide is lying down and interacting rather strongly with the treated glass. Addition of alcohol tended to decrease adsorption on the uncharged surfaces and to increase it on the charged amino-propyl surface.

UR - https://www.scopus.com/pages/publications/0030679449

M3 - Conference article

AN - SCOPUS:0030679449

SN - 0743-0515

VL - 76

SP - 546

JO - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering

JF - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering

T2 - Proceedings of the 1997 Spring ACS Meeting

Y2 - 13 April 1997 through 17 April 1997

ER -

Fundamental studies of DNA adsorption and hybridization on solid surfaces

Abstract

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