TY - JOUR
T1 - Fundamental studies of DNA adsorption and hybridization on solid surfaces
AU - Graves, David J.
AU - Sanguedolce, Lynn Anne
AU - Chan, Vincent
AU - McKenzie, Steven E.
AU - Surrey, Saul
AU - Fortina, Paolo
PY - 1997
Y1 - 1997
N2 - Results are presented of fundamental studies of DNA adsorption and hybridization on solid surfaces. Fluorescence recovery after photobleaching (FRAP) is used to study the intrinsic desorption kinetics for oligonucleotides adsorbed on silanized glass. The results suggest, that are in agreement with the work of others, that the nucleotide is lying down and interacting rather strongly with the treated glass. Addition of alcohol tended to decrease adsorption on the uncharged surfaces and to increase it on the charged amino-propyl surface.
AB - Results are presented of fundamental studies of DNA adsorption and hybridization on solid surfaces. Fluorescence recovery after photobleaching (FRAP) is used to study the intrinsic desorption kinetics for oligonucleotides adsorbed on silanized glass. The results suggest, that are in agreement with the work of others, that the nucleotide is lying down and interacting rather strongly with the treated glass. Addition of alcohol tended to decrease adsorption on the uncharged surfaces and to increase it on the charged amino-propyl surface.
UR - http://www.scopus.com/inward/record.url?scp=0030679449&partnerID=8YFLogxK
M3 - Conference article
AN - SCOPUS:0030679449
SN - 0743-0515
VL - 76
SP - 546
JO - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
JF - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
T2 - Proceedings of the 1997 Spring ACS Meeting
Y2 - 13 April 1997 through 17 April 1997
ER -