TY - JOUR
T1 - Fabrication of large-area ordered and reproducible nanostructures for SERS biosensor application
AU - Das, Gobind
AU - Patra, Niranjan
AU - Gopalakrishnan, Anisha
AU - Zaccaria, Remo Proietti
AU - Toma, Andrea
AU - Thorat, Sanjay
AU - Di Fabrizio, Enzo
AU - Diaspro, Alberto
AU - Salerno, Marco
PY - 2012/4/21
Y1 - 2012/4/21
N2 - We propose a large-area SERS device with efficient fluorescence quenching capability. The substrate is based on anodic porous alumina templates with various pore size and wall thickness as small as 15 and 36 nm, respectively. The nano-patterned SERS substrate, with excellent control and reproducibility of plasmon-polaritons generation, shows very efficient enhanced Raman signal in the presence of intrinsically fluorescent molecules such as cresyl violet, rhodamine, and green fluorescent protein. This work demonstrates that, when the nanostructures are properly designed and fabricated, Raman and fluorescence spectroscopy can be used in combination in order to obtain complementary molecular informations. Theoretical simulation shows excellent agreement with the experimental findings. The enhancement factor is found to be 10 3-104, with respect to flat gold surface when the molecules are supposed to be closely packed, with considerable fluorescence suppression, showing a promising disposable biosensor.
AB - We propose a large-area SERS device with efficient fluorescence quenching capability. The substrate is based on anodic porous alumina templates with various pore size and wall thickness as small as 15 and 36 nm, respectively. The nano-patterned SERS substrate, with excellent control and reproducibility of plasmon-polaritons generation, shows very efficient enhanced Raman signal in the presence of intrinsically fluorescent molecules such as cresyl violet, rhodamine, and green fluorescent protein. This work demonstrates that, when the nanostructures are properly designed and fabricated, Raman and fluorescence spectroscopy can be used in combination in order to obtain complementary molecular informations. Theoretical simulation shows excellent agreement with the experimental findings. The enhancement factor is found to be 10 3-104, with respect to flat gold surface when the molecules are supposed to be closely packed, with considerable fluorescence suppression, showing a promising disposable biosensor.
UR - http://www.scopus.com/inward/record.url?scp=84860260508&partnerID=8YFLogxK
U2 - 10.1039/c2an16022f
DO - 10.1039/c2an16022f
M3 - Article
AN - SCOPUS:84860260508
SN - 0003-2654
VL - 137
SP - 1785
EP - 1792
JO - Analyst
JF - Analyst
IS - 8
ER -