Extractive crystallization liquid biphasic system: Extractive crystallization

Revathy Sankaran, Kaoshilya Bai Narenthra Rao, Tau Chuan Ling, Pau Loke Show

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


The incorporation of a crystallization process into liquid biphasic systems (LBSs) has opened up avenues for the extraction of various compounds, especially in the biopharmaceutical field, such as antibodies, proteins, and more. The process refines the quality of crystals into valuable high-purity yields. In this chapter, the methods of inducing crystallization into liquid biphasic systems are discussed along with the potential volatile process conditions that could adversely affect the crystal growth, size, and morphology. The unpredictable nature of the nucleation process is discussed at length in terms of temperature; concentration of solvent, anti-solvent, and proteins, and pH of the system. The foreseeable potential of extractive crystallization in LBSs is discussed primarily for the lab scale and its potent industrial applications. Recent strides in overcoming the method’s shortcomings are also discussed.

Original languageBritish English
Title of host publicationLiquid Biphasic System
Subtitle of host publicationFundamentals and Applications in Bioseparation Technology
Number of pages21
ISBN (Electronic)9780128199862
StatePublished - 1 Jan 2020


  • Anti-solvent
  • Concentration
  • Crystallization
  • Liquid biphasic system
  • Nucleating agents
  • Seeding
  • Temperature


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