Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system

Hui Suan Ng; Chien Wei Ooi; Mohd Noriznan Mokhtar; Pau Loke Show; Arbakariya Ariff; Joo Shun Tan; Eng Poh Ng; Tau Chuan Ling

doi:10.1016/j.biortech.2013.05.087

Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system

Hui Suan Ng, Chien Wei Ooi, Mohd Noriznan Mokhtar, Pau Loke Show, Arbakariya Ariff, Joo Shun Tan, Eng Poh Ng, Tau Chuan Ling

Chemical and Petroleum Engineering

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (V_R) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8h. An average total CDs concentration of 13.7mg/mL, (4.77mg/mLα-CD, 5.02mg/mLβ-CD and 3.91mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase.

Original language	British English
Pages (from-to)	723-726
Number of pages	4
Journal	Bioresource Technology
Volume	142
DOIs	https://doi.org/10.1016/j.biortech.2013.05.087
State	Published - Aug 2013

Keywords

Aqueous two-phase system
Bacillus cereus
Cyclodextrin
Cyclodextrin glycosyltransferase
Extractive bioconversion

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2013.05.087

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title = "Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system",

abstract = "An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8h. An average total CDs concentration of 13.7mg/mL, (4.77mg/mLα-CD, 5.02mg/mLβ-CD and 3.91mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase.",

keywords = "Aqueous two-phase system, Bacillus cereus, Cyclodextrin, Cyclodextrin glycosyltransferase, Extractive bioconversion",

author = "Ng, {Hui Suan} and Ooi, {Chien Wei} and Mokhtar, {Mohd Noriznan} and Show, {Pau Loke} and Arbakariya Ariff and Tan, {Joo Shun} and Ng, {Eng Poh} and Ling, {Tau Chuan}",

note = "Funding Information: This work is supported financially by eScience fund ( 02-01-03-SF0649 ) from the Ministry of Science, Technology and Innovation (MOSTI) , Malaysia.",

year = "2013",

month = aug,

doi = "10.1016/j.biortech.2013.05.087",

language = "British English",

volume = "142",

pages = "723--726",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier",

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TY - JOUR

T1 - Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system

AU - Ng, Hui Suan

AU - Ooi, Chien Wei

AU - Mokhtar, Mohd Noriznan

AU - Show, Pau Loke

AU - Ariff, Arbakariya

AU - Tan, Joo Shun

AU - Ng, Eng Poh

AU - Ling, Tau Chuan

N1 - Funding Information: This work is supported financially by eScience fund ( 02-01-03-SF0649 ) from the Ministry of Science, Technology and Innovation (MOSTI) , Malaysia.

PY - 2013/8

Y1 - 2013/8

N2 - An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8h. An average total CDs concentration of 13.7mg/mL, (4.77mg/mLα-CD, 5.02mg/mLβ-CD and 3.91mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase.

AB - An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8h. An average total CDs concentration of 13.7mg/mL, (4.77mg/mLα-CD, 5.02mg/mLβ-CD and 3.91mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase.

KW - Aqueous two-phase system

KW - Bacillus cereus

KW - Cyclodextrin

KW - Cyclodextrin glycosyltransferase

KW - Extractive bioconversion

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M3 - Article

AN - SCOPUS:84879840793

SN - 0960-8524

VL - 142

SP - 723

EP - 726

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system

Abstract

Keywords

UN SDGs

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