Abstract
1. 1. The glutathione S-transferases of the following fungal species have been affinity purified on glutathione-Sepharose; Yarrowia lipolytica (NCYC825), Sporotrichum thermophile (ATCC16479), Talaromyces emersonii (ATCC26914) and Issatchenkia orientalis (CBS5147 and an isolate provided by Prof. Kumagai, Kyoyo University). 2. 2. SDS-PAGE analysis of affinity of purified fungal extracts, followed by Western blots with polyclonal antisera to rat GSTs, was performed and indicated the presence of Alpha and Mu class glutathione S-transferases in the fungi immunologically and electrophoretically similar to the rat enzymes. 3. 3. Each extract gave a characteristics affinity-gradient elution pattern on glutathione-agarose.
| Original language | British English |
|---|---|
| Pages (from-to) | 7-13 |
| Number of pages | 7 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 104 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1993 |