Evidence for Alpha and Mu class glutathione S-transferases in a number of fungal species

David Sheehan, James P. Casey

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

1. 1. The glutathione S-transferases of the following fungal species have been affinity purified on glutathione-Sepharose; Yarrowia lipolytica (NCYC825), Sporotrichum thermophile (ATCC16479), Talaromyces emersonii (ATCC26914) and Issatchenkia orientalis (CBS5147 and an isolate provided by Prof. Kumagai, Kyoyo University). 2. 2. SDS-PAGE analysis of affinity of purified fungal extracts, followed by Western blots with polyclonal antisera to rat GSTs, was performed and indicated the presence of Alpha and Mu class glutathione S-transferases in the fungi immunologically and electrophoretically similar to the rat enzymes. 3. 3. Each extract gave a characteristics affinity-gradient elution pattern on glutathione-agarose.

Original languageBritish English
Pages (from-to)7-13
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume104
Issue number1
DOIs
StatePublished - Jan 1993

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