Evaluating Self-buffering Ionic Liquids for Biotechnological Applications

Sze Ying Lee, Filipa A. Vicente, Francisca A. E Silva, Taînia E. Sintra, Mohamed Taha, Ianatul Khoiroh, João A.P. Coutinho, Pau Loke Show, Sónia P.M. Ventura

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

A new range of Good's buffer ionic liquids (GB-ILs), displaying simultaneously the properties of ionic liquids and Good's buffers, were synthesized by a combination of Good's buffer anions (MOPSO, BES, TAPSO, and CAPSO) and tetrabutylammonium, tetrabutylphosphonium, and cholinium cations via an acid-base neutralization reaction. The activity and stability of a lipolytic enzyme from Pseudomonas cepacia in aqueous solutions of these buffers were evaluated, and the results show their advantage as media for enzymatic reactions when compared to conventional phosphate buffers. Moreover aqueous biphasic systems (ABS) composed by these GB-ILs and potassium citrate were investigated and shown to be highly effective and selective for the partitioning of the lipolytic enzyme into the GB-IL-rich phase. The results allow for the development of an efficient and biocompatible process combining the self-buffering and enzyme-stabilizing properties of the GB-ILs in the reaction step, with the advantages of GB-ILs as extraction solvents in ABS.

Original languageBritish English
Pages (from-to)3420-3428
Number of pages9
JournalACS Sustainable Chemistry and Engineering
Volume3
Issue number12
DOIs
StatePublished - 27 Oct 2015

Keywords

  • Aqueous biphasic systems
  • Biocatalysis and bioseparation
  • Enzyme-stabilizing systems
  • Good's buffer ionic liquids
  • Self-buffering GB-ILs

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