Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule

James Murray, Jaehwan Sim, Kyunghoon Oh, Gihyun Sung, Ara Lee, Annadka Shrinidhi, Ayyavu Thirunarayanan, Dinesh Shetty, Kimoon Kim

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Chemical proteomics relies primarily on click-chemistry-based protein labeling and biotin-streptavidin enrichment, but these techniques have inherent limitations. Enrichment of intracellular proteins using a totally synthetic host–guest complex is described, overcoming the problem associated with the classical approach. We achieve this by affinity-based protein labeling with a target-specific probe molecule conjugated to a high-affinity guest (suberanilohydroxamic acid–ammonium-adamantane; SAHA-Ad) and then enriching the labeled species using a cucurbit[7]uril bead. This method shows high specificity for labeled molecules in a MDA-MB-231 breast cancer cell lysate. Moreover, this method shows promise for labeling proteins in live cells.

Original languageBritish English
Pages (from-to)2395-2398
Number of pages4
JournalAngewandte Chemie - International Edition
Volume56
Issue number9
DOIs
StatePublished - 20 Feb 2017

Keywords

  • cucurbiturils
  • host–guest chemistry
  • protein chemistry
  • protein enrichment
  • supramolecular chemistry

Fingerprint

Dive into the research topics of 'Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule'. Together they form a unique fingerprint.

Cite this