Abstract
In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10−2–2.04 × 10−1 mL/min·cm2) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.
Original language | British English |
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Pages (from-to) | 615-628 |
Number of pages | 14 |
Journal | International Journal of Biological Macromolecules |
Volume | 132 |
DOIs | |
State | Published - 1 Jul 2019 |
Keywords
- C-phycocyanin
- Chitosan-modified nanofiber membrane
- Negative chromatography
- Purity
- Spirulina platensis