Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane

I. Son Ng, Malcolm S.Y. Tang, Pau Loke Show, Zih Ming Chiou, Jung Chin Tsai, Yu Kaung Chang

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10−2–2.04 × 10−1 mL/min·cm2) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.

Original languageBritish English
Pages (from-to)615-628
Number of pages14
JournalInternational Journal of Biological Macromolecules
Volume132
DOIs
StatePublished - 1 Jul 2019

Keywords

  • C-phycocyanin
  • Chitosan-modified nanofiber membrane
  • Negative chromatography
  • Purity
  • Spirulina platensis

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