Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus

Badreddine Sellami; Hela Louati; Mohamed Dellali; Patricia Aissa; Ezzeddine Mahmoudi; Ana Varela Coelho; David Sheehan

doi:10.1007/s11356-013-2404-4

Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus

Badreddine Sellami, Hela Louati, Mohamed Dellali, Patricia Aissa, Ezzeddine Mahmoudi, Ana Varela Coelho, David Sheehan

Chemistry

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The effects of permethrin (PER) on a panel of antoxidant enzymes; superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and indices of protein oxidation status (carbonylation and free thiols) were determined in digestive gland and gills of the clam Ruditapes decussatus. Animals were exposed to 100 ppb PER for 2 days. These enzyme activities increased significantly in digestive gland (p < 0.05) after PER treatment and oxidative modification of proteins was detected in both gill and digestive gland extracts using redox proteomics. PER exposure significantly reduced the amount of protein free thiol groups in digestive gland rather than in gill, when compared to controls. Conversely, digestive gland showed significantly higher levels of carbonylated proteins than gill after PER exposure. Some proteins were successfully identified by mass spectrometry of tryptic peptides. Our data suggest that digestive gland of R. decussatus can be used as a model tissue for investigating environmental risk of PER contamination.

Original language	British English
Pages (from-to)	4461-4472
Number of pages	12
Journal	Environmental Science and Pollution Research
Volume	21
Issue number	6
DOIs	https://doi.org/10.1007/s11356-013-2404-4
State	Published - Mar 2014

Keywords

Oxidative stress
Permethrin
Redox proteomics
Ruditapes decussatus

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title = "Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus",

abstract = "The effects of permethrin (PER) on a panel of antoxidant enzymes; superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and indices of protein oxidation status (carbonylation and free thiols) were determined in digestive gland and gills of the clam Ruditapes decussatus. Animals were exposed to 100 ppb PER for 2 days. These enzyme activities increased significantly in digestive gland (p < 0.05) after PER treatment and oxidative modification of proteins was detected in both gill and digestive gland extracts using redox proteomics. PER exposure significantly reduced the amount of protein free thiol groups in digestive gland rather than in gill, when compared to controls. Conversely, digestive gland showed significantly higher levels of carbonylated proteins than gill after PER exposure. Some proteins were successfully identified by mass spectrometry of tryptic peptides. Our data suggest that digestive gland of R. decussatus can be used as a model tissue for investigating environmental risk of PER contamination.",

keywords = "Oxidative stress, Permethrin, Redox proteomics, Ruditapes decussatus",

author = "Badreddine Sellami and Hela Louati and Mohamed Dellali and Patricia Aissa and Ezzeddine Mahmoudi and Coelho, {Ana Varela} and David Sheehan",

year = "2014",

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AU - Sellami, Badreddine

AU - Louati, Hela

AU - Dellali, Mohamed

AU - Aissa, Patricia

AU - Mahmoudi, Ezzeddine

AU - Coelho, Ana Varela

AU - Sheehan, David

PY - 2014/3

Y1 - 2014/3

N2 - The effects of permethrin (PER) on a panel of antoxidant enzymes; superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and indices of protein oxidation status (carbonylation and free thiols) were determined in digestive gland and gills of the clam Ruditapes decussatus. Animals were exposed to 100 ppb PER for 2 days. These enzyme activities increased significantly in digestive gland (p < 0.05) after PER treatment and oxidative modification of proteins was detected in both gill and digestive gland extracts using redox proteomics. PER exposure significantly reduced the amount of protein free thiol groups in digestive gland rather than in gill, when compared to controls. Conversely, digestive gland showed significantly higher levels of carbonylated proteins than gill after PER exposure. Some proteins were successfully identified by mass spectrometry of tryptic peptides. Our data suggest that digestive gland of R. decussatus can be used as a model tissue for investigating environmental risk of PER contamination.

AB - The effects of permethrin (PER) on a panel of antoxidant enzymes; superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and indices of protein oxidation status (carbonylation and free thiols) were determined in digestive gland and gills of the clam Ruditapes decussatus. Animals were exposed to 100 ppb PER for 2 days. These enzyme activities increased significantly in digestive gland (p < 0.05) after PER treatment and oxidative modification of proteins was detected in both gill and digestive gland extracts using redox proteomics. PER exposure significantly reduced the amount of protein free thiol groups in digestive gland rather than in gill, when compared to controls. Conversely, digestive gland showed significantly higher levels of carbonylated proteins than gill after PER exposure. Some proteins were successfully identified by mass spectrometry of tryptic peptides. Our data suggest that digestive gland of R. decussatus can be used as a model tissue for investigating environmental risk of PER contamination.

KW - Oxidative stress

KW - Permethrin

KW - Redox proteomics

KW - Ruditapes decussatus

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U2 - 10.1007/s11356-013-2404-4

DO - 10.1007/s11356-013-2404-4

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AN - SCOPUS:84895890285

SN - 0944-1344

VL - 21

SP - 4461

EP - 4472

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

IS - 6

ER -

Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus

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