Effects of cryopreservation and density-gradient washing on phospholipase C zeta concentrations in human spermatozoa

Junaid Kashir, Ariane Heynen, Celine Jones, Claire Durrans, Jo Craig, Joaquín Gadea, Karen Turner, John Parrington, Kevin Coward

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Cryopreservation and density-gradient washing (DGW) are routinely used in infertility treatment. This study used quantitative immunofluorescence analysis to report how these techniques affect concentrations of the oocyte activation factor, phospholipase C zeta (PLCζ) in spermatozoa from fertile men. DGW significantly elevated the proportion of spermatozoa in which PLCζ could be detected (by 25-81%; P < 0.0001). In contrast, in four donors, cryopreservation significantly reduced PLCζ concentrations (by 20-56%; P < 0.0001). These findings indicate that while DGW positively selects spermatozoa with detectable PLCζ, cryopreservation has significant detrimental effects upon PLCζ concentrations. Since reduced PLCζ concentrations have been implicated in deficient oocyte activation and infertility, further study is highly warranted.

Original languageBritish English
Pages (from-to)263-267
Number of pages5
JournalReproductive BioMedicine Online
Volume23
Issue number2
DOIs
StatePublished - Aug 2011

Keywords

  • assisted reproductive technology (ART)
  • cryopreservation
  • density-gradient washing
  • oocyte activation
  • phospholipase C zeta (PLCζ)
  • spermatozoa

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