TY - JOUR
T1 - Effect of serum from patients with minimal to mild endometriosis on mouse embryo development in vitro
AU - Damewood, M. D.
AU - Hesla, J. S.
AU - Schlaff, W. D.
AU - Hubbard, M.
AU - Gearhart, J. D.
AU - Rock, J. A.
N1 - Funding Information:
This work was supported in part by grant No 1212/81 from the 'Comisi6n Asesora de Investigaci6n Cientffica y T6cnica" of the Ministerio d_e Educaci6n y Ciencia. Address correspondence to: Dr. J. M. S~inchezT apias, Liver Unit, Hospital Clinico y Provincial, Casanova. 143. Barcelona-36. Spain, Tel. (2) 53 42 44.
PY - 1990
Y1 - 1990
N2 - Two-cell mouse embryos were cultured at 37°C in 5% CO2, 95% air with a 7.5% serum supplement from patients with minimal to mild endometriosis, (group I, n = 31), tubal factor (group II, n = 33), male factor (group III, n = 17), fetal cord samples (group IV, n = 37), and Ham's F-10 medium (Gibco, Grand Island, NY) without a serum supplement (group V, n = 30). The progression to blastocyst stage (mean percent ± SE) at 96 hours in groups I, II, III, IV, and V was 29.9% ± 3.7%, 60.6% ± 4.9%, 56.2% ± 5.2%, 61.7% ± 5.8%, and 63.2% ± 6.9%, respectively. Serum factors appear to be associated with an inhibition of early embryogenesis, which may explain the decreased fertility rates observed in patients with minimal to mild endometriosis.
AB - Two-cell mouse embryos were cultured at 37°C in 5% CO2, 95% air with a 7.5% serum supplement from patients with minimal to mild endometriosis, (group I, n = 31), tubal factor (group II, n = 33), male factor (group III, n = 17), fetal cord samples (group IV, n = 37), and Ham's F-10 medium (Gibco, Grand Island, NY) without a serum supplement (group V, n = 30). The progression to blastocyst stage (mean percent ± SE) at 96 hours in groups I, II, III, IV, and V was 29.9% ± 3.7%, 60.6% ± 4.9%, 56.2% ± 5.2%, 61.7% ± 5.8%, and 63.2% ± 6.9%, respectively. Serum factors appear to be associated with an inhibition of early embryogenesis, which may explain the decreased fertility rates observed in patients with minimal to mild endometriosis.
UR - http://www.scopus.com/inward/record.url?scp=0025649207&partnerID=8YFLogxK
U2 - 10.1016/s0015-0282(16)53956-9
DO - 10.1016/s0015-0282(16)53956-9
M3 - Article
C2 - 2226927
AN - SCOPUS:0025649207
SN - 0015-0282
VL - 54
SP - 917
EP - 920
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 5
ER -