Downstream processing of virus-like particles with aqueous two-phase systems: Applications and challenges

Hui Yi Leong, Xiao Qian Fu, Pau Loke Show, Shan Jing Yao, Dong Qiang Lin

Research output: Contribution to journalReview articlepeer-review

8 Scopus citations


The advancement of recombinant virus-like particle-based vaccines has attracted global attention owing to substantially safety and high efficacy in provoking a protective immunity against various chronic and infectious diseases in humans and animals. A robust, low-cost, and scalability separation and purification technology is of utmost importance in the downstream processing of recombinant virus-like particles to produce affordable and safe vaccines. Being a relatively simple, environmentally friendly, and efficient biomolecules recovery approach, aqueous two-phase systems have received great attention from researchers worldwide. This review aims to highlight the challenges and outlook in addition to the current applications of aqueous two-phase systems in downstream processing of virus-like particles. The efforts will confidently reinforce scholars’ knowledge and fill in the valuable research gap in the aspect of concerning recombinant virus-like particle-based vaccines development, particularly related to the virus-like particles downstream production processes.

Original languageBritish English
Pages (from-to)2064-2076
Number of pages13
JournalJournal of Separation Science
Issue number12
StatePublished - Jun 2022


  • aqueous two-phase systems
  • continuous bio-manufacturing
  • extraction
  • integrated processes
  • virus-like particles


Dive into the research topics of 'Downstream processing of virus-like particles with aqueous two-phase systems: Applications and challenges'. Together they form a unique fingerprint.

Cite this