Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat

F. C. Howarth, A. Adem, E. A. Adeghate, N. A. Al Ali, A. M. Al Bastaki, F. R. Sorour, R. O. Hammoudi, N. A. Ghaleb, N. J. Chandler, H. Dobrzynski

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The distribution of atrial natriuretic peptide (ANP) in blood plasma and cardiac muscle and its effects on ventricular myocyte contraction and intracellular free calcium concentration [Ca2+]i in the streptozotocin (STZ)-induced diabetic rat have been investigated. Blood plasma concentration and heart atrial and ventricular contents of ANP were significantly increased in STZ-treated rats compared to age-matched controls. STZ treatment increased the number of ventricular myocytes immunolabeled with antibodies against ANP. In control myocytes the percentage of cells that labeled positively and negatively were 17% versus 83%, respectively. However, in myocytes from STZ-treated rat the percentages were 52% versus 53%. Time to peak (TPK) shortening was significantly and characteristically prolonged in myocytes from STZ-treated rats (360 ± 5 ms) compared to controls (305 ± 5 ms). Amplitude of the Ca2+ transient was significantly increased in myocytes from STZ-treated rats compared to controls (0.39 ± 0.02 versus 0.29 ± 0.02 fura-2 RU in controls) and treatment with ANP reduced the amplitude of the Ca2+ transient to control levels. ANP may have a protective role in STZ-induced diabetic rat heart.

Original languageBritish English
Pages (from-to)691-700
Number of pages10
JournalPeptides
Volume26
Issue number4
DOIs
StatePublished - Apr 2005

Keywords

  • Atrial natriuretic peptide
  • Calcium
  • Diabetes
  • Ventricular myocytes

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