Disposable electrochemical immunosensor array for the multiplexed detection of the drug metabolites morphine, tetrahydrocannabinol and benzoylecgonine

Shimaa Eissa, Rema A. Almthen, Mohammed Zourob

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Heroin, marijuana and cocaine are widely abused drugs. Their use can be readily detected by analyzing urine for the metabolites morphine (MOR), tetrahydrocannabinol (THC) or benzoylecgonine (BZC). A multiplex immunosensor is described here for detection of MOR, THC and BZC using screen printed carbon array electrodes modified with gold nanoparticles. Antibodies against MOR, THC and BZC were immobilized on eight electrodes in a sensor array simultaneously, and a competitive assay was used for the detection. The free analytes in the sample compete with bovine serum albumin-conjugated analytes for the immobilized antibodies on the sensor surface. The array is capable of detecting the three drugs simultaneously within 20–40 min. The method has a high sensitivity, with detection limits as low as 1.2, 7.0, and 8.0 pg.mL−1 for MOR, THC and BZC, respectively. Cross reactivity testing was preformed to monitor any nonspecific binding. The results revealed good selectivity. Urine samples were spiked with the 3 drugs and tested with the multiplexed immunosensor. Recovery percentages ranged between 88 to 115%. [Figure not available: see fulltext.].

Original languageBritish English
Article number523
JournalMicrochimica Acta
Volume186
Issue number8
DOIs
StatePublished - 1 Aug 2019

Keywords

  • Benzoylecgonine
  • Immunosensor
  • Morphine
  • Multiplexed detection
  • Tetrahydrocannabinol
  • Voltammetry

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