Discovery of α-Glucosidase Inhibitors from Marine Microorganisms: Optimization of Culture Conditions and Medium Composition

Nguyen Thi Hien Trang, Doris Ying Ying Tang, Kit Wayne Chew, Nguyen Thi Linh, Le Thanh Hoang, Nguyen Tien Cuong, Hoang Thi Yen, Nguyen Thi Thao, Nguyen Thi Trung, Pau Loke Show, Do Thi Tuyen

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Various studies showed that the suppression of α-glucosidase activity can impede the glucose absorption in our body, and therefore, it can be used to treat type 2 diabetes. Hence, the compounds with anti-α-glucosidase have gained considerable attention because of their potential application in diabetes treatment. In previous literature studies, these anti-α-glucosidase compounds were extracted from plants and fungus. Less studies are being conducted to identify the anti-α-glucosidase compounds in the microbial community. In this study, 23 marine bacterial strains were screened for their potential to suppress the α-glucosidase activity. The highest inhibitory activity was exhibited by isolated L06 which was identified as Oceanimonas smirnovii EBL6. The cultivation conditions, such as temperature and pH, were optimized to increase the production of α-glucosidase inhibitors by Oceanimonas smirnovii EBL6 strain. The result findings showed that the highest yield of α-glucosidase inhibitors can be obtained at the culture time of 120 h, fermentation temperature of 30 °C, and pH 4.6. Under these conditions, the inhibitory activity of α-glucosidase can reach 81%. The IC50 of n-butanol extract was 13.89 μg/ml, while standard acarbose was 31.16 μg/ml. Overall, these findings suggest that Oceanimonas smirnovii produces α-glucosidase inhibitors and could been applied in the biochemical and medicinal fields in the future.

Original languageBritish English
Pages (from-to)1004-1015
Number of pages12
JournalMolecular Biotechnology
Volume63
Issue number11
DOIs
StatePublished - Nov 2021

Keywords

  • Cultivation
  • Diabetes mellitus
  • Marine bacteria
  • Oceanimonas smirnovii EBL6
  • Therapeutic
  • α-glucosidase inhibitor

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