Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Yu Kiat Lin, Pau Loke Show, Yee Jiun Yap, Chin Ping Tan, Eng Poh Ng, Arbakariya B. Ariff, Mohamad Suffian B. Mohamad Annuar, Tau Chuan Ling

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (VR), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, VR of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (PFT) of 21.8 and a yield (YT) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF.

Original languageBritish English
Pages (from-to)684-689
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume120
Issue number6
DOIs
StatePublished - Dec 2015

Keywords

  • Aqueous two-phase flotation
  • Bacillus cereus
  • Cyclodextringlycosyltransferase
  • Polyethylene glycol-salt system
  • Purification

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