TY - JOUR
T1 - Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
AU - Lin, Yu Kiat
AU - Show, Pau Loke
AU - Yap, Yee Jiun
AU - Tan, Chin Ping
AU - Ng, Eng Poh
AU - Ariff, Arbakariya B.
AU - Mohamad Annuar, Mohamad Suffian B.
AU - Ling, Tau Chuan
N1 - Funding Information:
This work is supported financially by Postgraduate Research Grant (PPP) – PG116-2012B from University of Malaya , Fundamental Research Grant Scheme ( FRGS/1/2013/SG05/UNIM/02/1 and FP005-2013B ) from Ministry of Education Malaysia and MOSTI-02-02-12-SF0256 from Ministry of Science, Technology and Innovation .
Publisher Copyright:
© 2015 The Society for Biotechnology, Japan.
PY - 2015/12
Y1 - 2015/12
N2 - Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (VR), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, VR of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (PFT) of 21.8 and a yield (YT) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF.
AB - Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (VR), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, VR of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (PFT) of 21.8 and a yield (YT) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF.
KW - Aqueous two-phase flotation
KW - Bacillus cereus
KW - Cyclodextringlycosyltransferase
KW - Polyethylene glycol-salt system
KW - Purification
UR - http://www.scopus.com/inward/record.url?scp=84946477044&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2015.04.013
DO - 10.1016/j.jbiosc.2015.04.013
M3 - Article
C2 - 26111602
AN - SCOPUS:84946477044
SN - 1389-1723
VL - 120
SP - 684
EP - 689
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 6
ER -