Direct electrochemistry of myoglobin at silver nanoparticles/myoglobin biocomposite: Application for hydrogen peroxide sensing

Selvakumar Palanisamy, Chelladurai Karuppiah, Shen Ming Chen, R. Emmanuel, P. Muthukrishnan, P. Prakash

    Research output: Contribution to journalArticlepeer-review

    51 Scopus citations

    Abstract

    Herein we report a novel hydrogen peroxide (H2O2) sensor based on direct electrochemistry of myoglobin at a silver nanoparticles/myoglobin (Ag-NPs/MB) biocomposite modified electrode. The green synthesized Ag-NPs and MB were used for the fabrication of Ag-NPs/MB biocomposite. The fabricated Ag-NPs and Ag-NPs/MB biocomposite were characterized by using TEM and SEM respectively. A good direct electron transfer of MB is realized at Ag-NPs/MB biocomposite electrode rather than direct drop casting of MB onto Ag-NPs modified electrode, which indicates that the native structure of MB is retained in the biocomposite modified electrode. The apparent heterogenous electron transfer rate constant (ks) is calculated as 3.02 s-1, which confirms a fast direct electron transfer of MB at the biocomposite electrode. A fast amperometric response for H2O 2 (<5 s) at Ag-NPs/MB biocomposite modified electrode indicates a good catalytic activity of the modified electrode. The H2O 2 detection is found in the linear response range from 1 μM to 3 mM with the detection limit of 0.088 μM. The sensitivity is calculated as 0.357 μA μM-1 cm-2. In addition, the fabricated biosensor shows a good consistency along with excellent precision.

    Original languageBritish English
    Pages (from-to)177-184
    Number of pages8
    JournalSensors and Actuators, B: Chemical
    Volume202
    DOIs
    StatePublished - 31 Oct 2014

    Keywords

    • Amperometry
    • Biocomposite
    • Direct electrochemistry
    • Hydrogen peroxide
    • Myoglobin
    • Silver nanoparticles

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