Direct Electrochemistry of Glucose Oxidase at Reduced Graphene Oxide and β-Cyclodextrin Composite Modified Electrode and Application for Glucose Biosensing

Selvakumar Palanisamy, Rajkumar Devasenathipathy, Shen Ming Chen, M. Ajmal Ali, Chelladurai Karuppiah, V. Balakumar, P. Prakash, Mohamed S. Elshikh, Fahad M.A. Al-Hemaid

    Research output: Contribution to journalArticlepeer-review

    23 Scopus citations

    Abstract

    A simple glucose biosensor has been developed based on direct electrochemistry of glucose oxidase (GOx) immobilized on the reduced graphene oxide (RGO) and β-cyclodextrin (CD) composite. A well-defined redox couple of GOx appears with a formal potential of ∼-0.459V at RGO/CD composite. A heterogeneous electron transfer rate constant (Ks) has been calculated for GOx at RGO/CD as 3.8s-1. The fabricated biosensor displays a wide response to glucose in the linear concentrations range from 50μM to 3.0mM. The sensitivity and limit of detection of the biosensor is estimated as 59.74μAmM-1cm-2 and 12μM, respectively.

    Original languageBritish English
    Pages (from-to)2412-2420
    Number of pages9
    JournalElectroanalysis
    Volume27
    Issue number10
    DOIs
    StatePublished - Oct 2015

    Keywords

    • Direct electrochemistry
    • Glucose biosensor
    • Glucose oxidase
    • Reduced graphene oxide
    • β-cyclodextrin

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