Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode

Selvakumar Palanisamy, Chelladurai Karuppiah, Shen Ming Chen

    Research output: Contribution to journalArticlepeer-review

    140 Scopus citations

    Abstract

    The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E°) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (Ks) of GOx at the nanocomposite was calculated to be 5.27s-1, revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5mM with a detection limit of 0.16mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose.

    Original languageBritish English
    Pages (from-to)164-169
    Number of pages6
    JournalColloids and Surfaces B: Biointerfaces
    Volume114
    DOIs
    StatePublished - 1 Feb 2014

    Keywords

    • Direct electron transfer
    • Glucose biosensor
    • Glucose oxidase
    • Reduced graphene oxide
    • Silver nanoparticles

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