TY - JOUR
T1 - Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode
AU - Palanisamy, Selvakumar
AU - Karuppiah, Chelladurai
AU - Chen, Shen Ming
N1 - Funding Information:
This project was supported by the National Science Council and the Ministry of Education of Taiwan (Republic of China) .
PY - 2014/2/1
Y1 - 2014/2/1
N2 - The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E°) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (Ks) of GOx at the nanocomposite was calculated to be 5.27s-1, revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5mM with a detection limit of 0.16mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose.
AB - The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E°) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (Ks) of GOx at the nanocomposite was calculated to be 5.27s-1, revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5mM with a detection limit of 0.16mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose.
KW - Direct electron transfer
KW - Glucose biosensor
KW - Glucose oxidase
KW - Reduced graphene oxide
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84886863017&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2013.10.006
DO - 10.1016/j.colsurfb.2013.10.006
M3 - Article
C2 - 24184536
AN - SCOPUS:84886863017
SN - 0927-7765
VL - 114
SP - 164
EP - 169
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -