Different regulatory patterns of M1 and M2 muscarinic receptor subtype RNA in SH-SY5Y human neuroblastoma induced by phorbol ester or DMSO

Ahmet Koman; Sylvie Cazaubon; Abdu Adem; Pierre Olivier Couraud; A. Donny Strosberg

doi:10.1016/0304-3940(93)90352-L

Different regulatory patterns of M₁ and M₂ muscarinic receptor subtype RNA in SH-SY5Y human neuroblastoma induced by phorbol ester or DMSO

Ahmet Koman, Sylvie Cazaubon, Abdu Adem, Pierre Olivier Couraud, A. Donny Strosberg

College of Medicine and Health Sciences

Institut Pasteur, Paris

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The human neuroblastoma cells SH-SY5Y were treated with the differentiation agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethyl sulfoxide (DMSO) and the muscarinic receptor subtype M₁ and M₂ RNA levels analyzed. After a decrease induced by both agents, the M₁ level gradually returned to normal in the presence of TPA but remained minimal with DMSO. As for M₂, several phases were observed with TPA, while DMSO caused a drastic increase. The data obtained with TPA were tentatively correlated with the amounts of immunoreactive PKCα. In conclusion, our results reveal: (a) differential regulation of M₁ and M₂ muscarinic receptor subtypes by either treatment; (b) opposing effects of TPA and DMSO on both subtypes.

Original language	British English
Pages (from-to)	79-82
Number of pages	4
Journal	Neuroscience Letters
Volume	149
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(93)90352-L
State	Published - 4 Jan 1993

Keywords

Differentiation
Dimethyl sulfoxide
Gene regulation
Muscarinic receptor
Phorbol ester
Protein kinase C
RNA
SH-SY5Y human neuroblastoma

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10.1016/0304-3940(93)90352-L

Cite this

@article{9ab69875eab5497381ce2d556c96e7bc,

title = "Different regulatory patterns of M1 and M2 muscarinic receptor subtype RNA in SH-SY5Y human neuroblastoma induced by phorbol ester or DMSO",

abstract = "The human neuroblastoma cells SH-SY5Y were treated with the differentiation agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethyl sulfoxide (DMSO) and the muscarinic receptor subtype M1 and M2 RNA levels analyzed. After a decrease induced by both agents, the M1 level gradually returned to normal in the presence of TPA but remained minimal with DMSO. As for M2, several phases were observed with TPA, while DMSO caused a drastic increase. The data obtained with TPA were tentatively correlated with the amounts of immunoreactive PKCα. In conclusion, our results reveal: (a) differential regulation of M1 and M2 muscarinic receptor subtypes by either treatment; (b) opposing effects of TPA and DMSO on both subtypes.",

keywords = "Differentiation, Dimethyl sulfoxide, Gene regulation, Muscarinic receptor, Phorbol ester, Protein kinase C, RNA, SH-SY5Y human neuroblastoma",

author = "Ahmet Koman and Sylvie Cazaubon and Abdu Adem and Couraud, {Pierre Olivier} and Strosberg, {A. Donny}",

note = "Funding Information: This work was supported by the Institut National de la Sant6 et de la Recherche Mrdicale, Medicinska Forsk-ningsradet MFR and Svenska Institutet SI, Sweden, The Council for Tobacco Research, USA, Association pour la Recherche sur le Cancer, Ministrre de la Recherche et de l'Enseignement Suprrieur, Ligue Fran~aise contre le Cancer and the Fondation pour la Recherche Mrdicale. A.A. was supported by an EMBO fellowship.",

year = "1993",

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doi = "10.1016/0304-3940(93)90352-L",

language = "British English",

volume = "149",

pages = "79--82",

journal = "Neuroscience Letters",

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T1 - Different regulatory patterns of M1 and M2 muscarinic receptor subtype RNA in SH-SY5Y human neuroblastoma induced by phorbol ester or DMSO

AU - Koman, Ahmet

AU - Cazaubon, Sylvie

AU - Adem, Abdu

AU - Couraud, Pierre Olivier

AU - Strosberg, A. Donny

N1 - Funding Information: This work was supported by the Institut National de la Sant6 et de la Recherche Mrdicale, Medicinska Forsk-ningsradet MFR and Svenska Institutet SI, Sweden, The Council for Tobacco Research, USA, Association pour la Recherche sur le Cancer, Ministrre de la Recherche et de l'Enseignement Suprrieur, Ligue Fran~aise contre le Cancer and the Fondation pour la Recherche Mrdicale. A.A. was supported by an EMBO fellowship.

PY - 1993/1/4

Y1 - 1993/1/4

N2 - The human neuroblastoma cells SH-SY5Y were treated with the differentiation agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethyl sulfoxide (DMSO) and the muscarinic receptor subtype M1 and M2 RNA levels analyzed. After a decrease induced by both agents, the M1 level gradually returned to normal in the presence of TPA but remained minimal with DMSO. As for M2, several phases were observed with TPA, while DMSO caused a drastic increase. The data obtained with TPA were tentatively correlated with the amounts of immunoreactive PKCα. In conclusion, our results reveal: (a) differential regulation of M1 and M2 muscarinic receptor subtypes by either treatment; (b) opposing effects of TPA and DMSO on both subtypes.

AB - The human neuroblastoma cells SH-SY5Y were treated with the differentiation agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethyl sulfoxide (DMSO) and the muscarinic receptor subtype M1 and M2 RNA levels analyzed. After a decrease induced by both agents, the M1 level gradually returned to normal in the presence of TPA but remained minimal with DMSO. As for M2, several phases were observed with TPA, while DMSO caused a drastic increase. The data obtained with TPA were tentatively correlated with the amounts of immunoreactive PKCα. In conclusion, our results reveal: (a) differential regulation of M1 and M2 muscarinic receptor subtypes by either treatment; (b) opposing effects of TPA and DMSO on both subtypes.

KW - Differentiation

KW - Dimethyl sulfoxide

KW - Gene regulation

KW - Muscarinic receptor

KW - Phorbol ester

KW - Protein kinase C

KW - RNA

KW - SH-SY5Y human neuroblastoma

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