Development of Aurantiochytrium limacinum SR21 cultivation using salt-rich waste feedstock for docosahexaenoic acid production and application of natural colourant in food product

Hui Yi Leong, Chien An Su, Bo Sheng Lee, John Chi Wei Lan, Chung Lim Law, Jo Shu Chang, Pau Loke Show

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Microalgae biorefinery is presently receiving a lot of attention as driven by its production of high value-added products. In this study, an oleaginous microalga Aurantiochytrium limacinum SR21 was cultured for docosahexaenoic acid (DHA) production using 20% (w/v) of K 2 HPO 4 -waste feedstock to replace 0.005% (w/v) of KH 2 PO 4 in the flask culture. DHA is an essential nutrient for human's brain functionalities. Collectively, the K 2 HPO 4 -waste feedstock with working concentration of 0.005% (w/v) in the cultivation prompted a higher lipid content (8.29%) and DHA production (128.81 mg.L −1 ). Moreover, natural plant pigment products containing stabilised betacyanins were utilised as natural red colourants for hard candy production. This study develops microalgal cultivation using salt-rich waste feedstock for a higher lipid and DHA content as well as application of natural colouring agents in food products.

Original languageBritish English
Pages (from-to)30-36
Number of pages7
JournalBioresource Technology
Volume271
DOIs
StatePublished - Jan 2019

Keywords

  • Aurantiochytrium limacinum SR21
  • Docosahexaenoic acid
  • Microalgae biorefinery
  • Natural colourant
  • Salt-rich waste feedstock

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