@article{a0e49fb360454ac3a2480ba585cc442e,
title = "Detection of Ganglioside-Specific Toxin Binding with Biomembrane-Based Bioelectronic Sensors",
abstract = "Gangliosides, glycolipids that are abundant in the plasma membrane outer leaflet, play an integral role in cellular recognition, adhesion, and infection by interacting with different endogenous molecules, viruses, and toxins. Model membrane systems, such as ganglioside-enriched supported lipid bilayers (SLBs), present a useful tool for sensing, characterizing, and quantifying such interactions. In this work, we report the formation of ganglioside GM1-rich SLBs on conducting polymer electrodes using a solvent-Assisted lipid bilayer assembly method to investigate changes in membrane electrical properties upon binding of the B subunit of cholera toxin. The sensing capabilities of our platform were investigated by varying both the receptor and the toxin concentrations in the system as well as using a complex sample (milk contaminated with the toxin) and monitoring the changes in the electrical properties of the membrane. Our work highlights the potential of such conducting polymer-supported biomembrane-based platforms for detecting the toxins within a complex environment, studying ganglioside-specific biomolecular interactions with toxins and screening inhibitory molecules to prevent these interactions.",
keywords = "cholera toxin, electrochemical impedance spectroscopy, ganglioside, PEDOT:PSS, supported bilayer",
author = "{Bint E Naser}, {Samavi Farnush} and Hui Su and Liu, {Han Yuan} and Manzer, {Zachary A.} and Zhongmou Chao and Arpita Roy and Pappa, {Anna Maria} and Alberto Salleo and Owens, {R{\'o}is{\'i}n M.} and Susan Daniel",
note = "Funding Information: We acknowledge funding for this project, sponsored by the Defense Advanced Research Projects Agency (DARPA) Army Research Office and accomplished under Cooperative Agreement Number W911NF-18-2-0152. ZAM acknowledges support by T32GM008500 from the National Institute of General Medical Sciences. Part of this work was performed in part at Cornell Nanoscale Facility (CNF), a member of the National Nanotechnology Coordinated Infrastructure (NNCI), which is supported by the National Science Foundation (NSF) (grant number NNCI-2025233). The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of DARPA or the Army Research Office, the National Science Foundation, the National Institute of General Medical Sciences, the National Institutes of Health, or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",
year = "2021",
month = nov,
day = "15",
doi = "10.1021/acsabm.1c00878",
language = "British English",
volume = "4",
pages = "7942--7950",
journal = "ACS Applied Bio Materials",
issn = "2576-6422",
publisher = "American Chemical Society",
number = "11",
}
