Detection of Ganglioside-Specific Toxin Binding with Biomembrane-Based Bioelectronic Sensors

Samavi Farnush Bint E Naser, Hui Su, Han Yuan Liu, Zachary A. Manzer, Zhongmou Chao, Arpita Roy, Anna Maria Pappa, Alberto Salleo, Róisín M. Owens, Susan Daniel

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Gangliosides, glycolipids that are abundant in the plasma membrane outer leaflet, play an integral role in cellular recognition, adhesion, and infection by interacting with different endogenous molecules, viruses, and toxins. Model membrane systems, such as ganglioside-enriched supported lipid bilayers (SLBs), present a useful tool for sensing, characterizing, and quantifying such interactions. In this work, we report the formation of ganglioside GM1-rich SLBs on conducting polymer electrodes using a solvent-Assisted lipid bilayer assembly method to investigate changes in membrane electrical properties upon binding of the B subunit of cholera toxin. The sensing capabilities of our platform were investigated by varying both the receptor and the toxin concentrations in the system as well as using a complex sample (milk contaminated with the toxin) and monitoring the changes in the electrical properties of the membrane. Our work highlights the potential of such conducting polymer-supported biomembrane-based platforms for detecting the toxins within a complex environment, studying ganglioside-specific biomolecular interactions with toxins and screening inhibitory molecules to prevent these interactions.

Original languageBritish English
Pages (from-to)7942-7950
Number of pages9
JournalACS Applied Bio Materials
Issue number11
StatePublished - 15 Nov 2021


  • cholera toxin
  • electrochemical impedance spectroscopy
  • ganglioside
  • supported bilayer


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