TY - JOUR
T1 - Detecting Arbitrary DNA Mutations Using Graphene Oxide and Ethidium Bromide
AU - Huang, Jiahao
AU - Wang, Zhenyu
AU - Kim, Jang Kyo
AU - Su, Xuefen
AU - Li, Zhigang
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/12/15
Y1 - 2015/12/15
N2 - We propose a simple and fast method for detecting arbitrary DNA mutations. Single-stranded DNA probes labeled with fluorescein amidite (FAM-ssDNA), ethidium bromide (EB), and graphene oxide (GO) are employed in the sensing system. The detection is achieved in two steps. In the first step, the sensing system contains FAM-ssDNA probes and EB molecules. It exhibits different fluorescence emissions in the presence of perfectly matched, mismatched, and random DNA sequences. With the addition of GO in the second step, the fluorescence signal for perfectly matched and random DNA does not vary greatly, which, however, experiences a significant change for mismatched DNA targets. The signal ratio before and after the addition of GO can clearly distinguish mutations from normal and random DNA sequences. The detection method works well regardless of the mutation positions and only requires "mix-and-detect" steps, which are completed within 15 min.
AB - We propose a simple and fast method for detecting arbitrary DNA mutations. Single-stranded DNA probes labeled with fluorescein amidite (FAM-ssDNA), ethidium bromide (EB), and graphene oxide (GO) are employed in the sensing system. The detection is achieved in two steps. In the first step, the sensing system contains FAM-ssDNA probes and EB molecules. It exhibits different fluorescence emissions in the presence of perfectly matched, mismatched, and random DNA sequences. With the addition of GO in the second step, the fluorescence signal for perfectly matched and random DNA does not vary greatly, which, however, experiences a significant change for mismatched DNA targets. The signal ratio before and after the addition of GO can clearly distinguish mutations from normal and random DNA sequences. The detection method works well regardless of the mutation positions and only requires "mix-and-detect" steps, which are completed within 15 min.
UR - http://www.scopus.com/inward/record.url?scp=84950105437&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.5b03369
DO - 10.1021/acs.analchem.5b03369
M3 - Article
C2 - 26559174
AN - SCOPUS:84950105437
SN - 0003-2700
VL - 87
SP - 12254
EP - 12261
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 24
ER -