Detecting Arbitrary DNA Mutations Using Graphene Oxide and Ethidium Bromide

Jiahao Huang, Zhenyu Wang, Jang Kyo Kim, Xuefen Su, Zhigang Li

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We propose a simple and fast method for detecting arbitrary DNA mutations. Single-stranded DNA probes labeled with fluorescein amidite (FAM-ssDNA), ethidium bromide (EB), and graphene oxide (GO) are employed in the sensing system. The detection is achieved in two steps. In the first step, the sensing system contains FAM-ssDNA probes and EB molecules. It exhibits different fluorescence emissions in the presence of perfectly matched, mismatched, and random DNA sequences. With the addition of GO in the second step, the fluorescence signal for perfectly matched and random DNA does not vary greatly, which, however, experiences a significant change for mismatched DNA targets. The signal ratio before and after the addition of GO can clearly distinguish mutations from normal and random DNA sequences. The detection method works well regardless of the mutation positions and only requires "mix-and-detect" steps, which are completed within 15 min.

Original languageBritish English
Pages (from-to)12254-12261
Number of pages8
JournalAnalytical Chemistry
Volume87
Issue number24
DOIs
StatePublished - 15 Dec 2015

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