TY - JOUR
T1 - Currently circulating genotypes of Hepatitis A virus in South West, East and North East India, 2017–2018
AU - Ameen, Alyusif
AU - Sabeena, Sasidharanpillai
AU - Robin, Sudandiradas
AU - Sanjay, Ramachandran
AU - Prasad, Varamballi
AU - Mevis, Fernandes M.
AU - Bhatt, Puneet
AU - Arunkumar, Govindakarnavar
N1 - Funding Information:
We acknowledge our sincere gratitude to the clinicians from different part of India. The study was partially supported by CDC Cooperative Agreement ( 5U01GH001051 ) and Indian Council of Medical Research (ICMR) grant, India (File No. 5/8/7/15/2010/ECD-I ).
Publisher Copyright:
© 2021
PY - 2022/4
Y1 - 2022/4
N2 - Background: This study was carried out to understand the circulating genotypes of Hepatitis A virus (HAV) in South West, East and North East India during the period 2017–2018 as a part of acute febrile illness surveillance at the Manipal Institute of Virology. Methods: Archived serum samples of 48 Hepatitis A confirmed cases were subjected to RNA extraction using QIAamp® Viral RNA Mini Kit (QIAGEN, Germany). The samples with molecular confirmation for HAV by reverse transcriptase real-Time PCR (Real Star® HAV RT-PCR Kit 2.0, Altona Diagnostics, GmbH, Hamburg, Germany) were further subjected to nested conventional PCR targeting the 5′ UTR region. The purified PCR products were sequenced using Big Dye Terminator Kit (Applied Biosystems, USA), in a 3500 XL genetic analyzer (Applied Biosystems, USA). The edited sequences by means of MEGA X (MEGA version 10.1) were compared with reference sequences in the NCBI nucleotide database. Results: From states of Assam, Goa, Gujarat, Karnataka, Kerala, Maharashtra, Odisha, Tamil Nadu and Tripura, 139 Hepatitis A and 33 Hepatitis E cases were reported during the study period. The median age of the acute Hepatitis A cases was 19 years (IQR 12.8–24) and most of the affected individuals were students between 10 and 19 years (52.5%). In the present study, 14 samples from Assam, Goa, Gujarat, Karnataka, Odisha, Kerala, Maharashtra and Tamil Nadu were genotyped as genotype IIIA by nested conventional polymerase chain reaction. Conclusion: The circulating HAV genotype in South West, North East and East India between 2017 and 2018 was IIIA.
AB - Background: This study was carried out to understand the circulating genotypes of Hepatitis A virus (HAV) in South West, East and North East India during the period 2017–2018 as a part of acute febrile illness surveillance at the Manipal Institute of Virology. Methods: Archived serum samples of 48 Hepatitis A confirmed cases were subjected to RNA extraction using QIAamp® Viral RNA Mini Kit (QIAGEN, Germany). The samples with molecular confirmation for HAV by reverse transcriptase real-Time PCR (Real Star® HAV RT-PCR Kit 2.0, Altona Diagnostics, GmbH, Hamburg, Germany) were further subjected to nested conventional PCR targeting the 5′ UTR region. The purified PCR products were sequenced using Big Dye Terminator Kit (Applied Biosystems, USA), in a 3500 XL genetic analyzer (Applied Biosystems, USA). The edited sequences by means of MEGA X (MEGA version 10.1) were compared with reference sequences in the NCBI nucleotide database. Results: From states of Assam, Goa, Gujarat, Karnataka, Kerala, Maharashtra, Odisha, Tamil Nadu and Tripura, 139 Hepatitis A and 33 Hepatitis E cases were reported during the study period. The median age of the acute Hepatitis A cases was 19 years (IQR 12.8–24) and most of the affected individuals were students between 10 and 19 years (52.5%). In the present study, 14 samples from Assam, Goa, Gujarat, Karnataka, Odisha, Kerala, Maharashtra and Tamil Nadu were genotyped as genotype IIIA by nested conventional polymerase chain reaction. Conclusion: The circulating HAV genotype in South West, North East and East India between 2017 and 2018 was IIIA.
KW - Genotypes
KW - HAV
KW - Hepatitis A virus
KW - India
KW - Viral hepatitis
UR - http://www.scopus.com/inward/record.url?scp=85101633400&partnerID=8YFLogxK
U2 - 10.1016/j.mjafi.2020.11.019
DO - 10.1016/j.mjafi.2020.11.019
M3 - Article
AN - SCOPUS:85101633400
SN - 0377-1237
VL - 78
SP - 185
EP - 191
JO - Medical Journal Armed Forces India
JF - Medical Journal Armed Forces India
IS - 2
ER -