Currently circulating genotypes of Hepatitis A virus in South West, East and North East India, 2017–2018

Alyusif Ameen, Sasidharanpillai Sabeena, Sudandiradas Robin, Ramachandran Sanjay, Varamballi Prasad, Fernandes M. Mevis, Puneet Bhatt, Govindakarnavar Arunkumar

    Research output: Contribution to journalArticlepeer-review

    3 Scopus citations

    Abstract

    Background: This study was carried out to understand the circulating genotypes of Hepatitis A virus (HAV) in South West, East and North East India during the period 2017–2018 as a part of acute febrile illness surveillance at the Manipal Institute of Virology. Methods: Archived serum samples of 48 Hepatitis A confirmed cases were subjected to RNA extraction using QIAamp® Viral RNA Mini Kit (QIAGEN, Germany). The samples with molecular confirmation for HAV by reverse transcriptase real-Time PCR (Real Star® HAV RT-PCR Kit 2.0, Altona Diagnostics, GmbH, Hamburg, Germany) were further subjected to nested conventional PCR targeting the 5′ UTR region. The purified PCR products were sequenced using Big Dye Terminator Kit (Applied Biosystems, USA), in a 3500 XL genetic analyzer (Applied Biosystems, USA). The edited sequences by means of MEGA X (MEGA version 10.1) were compared with reference sequences in the NCBI nucleotide database. Results: From states of Assam, Goa, Gujarat, Karnataka, Kerala, Maharashtra, Odisha, Tamil Nadu and Tripura, 139 Hepatitis A and 33 Hepatitis E cases were reported during the study period. The median age of the acute Hepatitis A cases was 19 years (IQR 12.8–24) and most of the affected individuals were students between 10 and 19 years (52.5%). In the present study, 14 samples from Assam, Goa, Gujarat, Karnataka, Odisha, Kerala, Maharashtra and Tamil Nadu were genotyped as genotype IIIA by nested conventional polymerase chain reaction. Conclusion: The circulating HAV genotype in South West, North East and East India between 2017 and 2018 was IIIA.

    Original languageBritish English
    Pages (from-to)185-191
    Number of pages7
    JournalMedical Journal Armed Forces India
    Volume78
    Issue number2
    DOIs
    StatePublished - Apr 2022

    Keywords

    • Genotypes
    • HAV
    • Hepatitis A virus
    • India
    • Viral hepatitis

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