TY - JOUR
T1 - Current trends in polyhydroxyalkanoates (PHAs) biosynthesis
T2 - Insights from the recombinant Escherichia coli
AU - Leong, Yoong Kit
AU - Show, Pau Loke
AU - Ooi, Chien Wei
AU - Ling, Tau Chuan
AU - Lan, John Chi Wei
N1 - Funding Information:
This work is supported financially by Fundamental Research Grant Scheme (Malaysia, FRGS/1/2013/SG05/UNIM/02/1 ), University of Nottingham Malaysia Campus Grant ( UNR30006 and Dean Scholarship) and by National Science Council (Taiwan, NSC102-2221-E-155-057 and NSC101-2632-E-155-001-MY3 ).
PY - 2014/6/20
Y1 - 2014/6/20
N2 - Pursuing the current trend, the "green-polymers", polyhydroxyalkanoates (PHAs) which are degradable and made from renewable sources have been a potential substitute for synthetic plastics. Due to the increasing concern towards escalating crude oil price, depleting petroleum resource and environmental damages done by plastics, PHAs have gained more and more attractions, both from industry and research. From the view point of Escherichia coli, a microorganism that used in the biopolymer large scale production, this paper describes the backgrounds of PHA and summarizes the current advances in PHA developments. In the short-chain-length (scl) PHAs section, the study of poly[(R)-3-hydroxybutyrate] [P(3HB)] as model polymer, ultra-high-molecular-weight P(3HB) which rarely discussed, and P(3HB-co-3HV), another commercialized PHA polymer are included. Other than that, this review also shed some light on the new members of PHA family, lactate-based PHAs and P(3HP) with topics such as block copolymers and invention of novel biopolymers. Flexibility of microorganisms in utilizing different carbon sources to accumulate medium-chain-length (mcl) PHAs and lastly, the promising scl-mcl-PHAs with interesting properties are also discussed.
AB - Pursuing the current trend, the "green-polymers", polyhydroxyalkanoates (PHAs) which are degradable and made from renewable sources have been a potential substitute for synthetic plastics. Due to the increasing concern towards escalating crude oil price, depleting petroleum resource and environmental damages done by plastics, PHAs have gained more and more attractions, both from industry and research. From the view point of Escherichia coli, a microorganism that used in the biopolymer large scale production, this paper describes the backgrounds of PHA and summarizes the current advances in PHA developments. In the short-chain-length (scl) PHAs section, the study of poly[(R)-3-hydroxybutyrate] [P(3HB)] as model polymer, ultra-high-molecular-weight P(3HB) which rarely discussed, and P(3HB-co-3HV), another commercialized PHA polymer are included. Other than that, this review also shed some light on the new members of PHA family, lactate-based PHAs and P(3HP) with topics such as block copolymers and invention of novel biopolymers. Flexibility of microorganisms in utilizing different carbon sources to accumulate medium-chain-length (mcl) PHAs and lastly, the promising scl-mcl-PHAs with interesting properties are also discussed.
KW - Escherichia coli
KW - Metabolic engineering
KW - Polyhydroxyalkanoates
UR - http://www.scopus.com/inward/record.url?scp=84899530091&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2014.03.020
DO - 10.1016/j.jbiotec.2014.03.020
M3 - Article
C2 - 24698847
AN - SCOPUS:84899530091
SN - 0168-1656
VL - 180
SP - 52
EP - 65
JO - Journal of Biotechnology
JF - Journal of Biotechnology
ER -
