Copper-pyridine-2,4,6-tricarboxylate metal organic framework nanoparticles based opto-electrochemical sensor for sensitive detection of matrix metalloproteinase‑2: Colloids and Surfaces A: Physicochemical and Engineering Aspects

M.A.H. Nawaz, S. Zulfiqar, M. Abbas, W. Zhou, Y. Li, W. Han, D.D. He, N. Liu, A. Hayat, M.H. Akhtar, C. Yu

Research output: Contribution to journalArticlepeer-review

Abstract

We designed a multiple signal sensing probe based on highly luminescent nanosized Cu-PTC metal-organic framework (MOF). The as synthesized nano-MOF was characterized by various techniques to confirm its morphology, crystallinity and elemental composition. A positively charged matrix metalloproteinase-2 (MMP-2) substrate (a polypeptide) was designed and prepared to interact electrostatically with the negatively charged Cu-PTC MOF probe, resulting in decrease in fluorescence response of the probe. Subsequently, MMP-2 was specifically recognized by the MMP-2 substrate and cleavage of the MMP-2 substrate resulted in “turn-on” fluorescence response from 1 to 175 ng/mL of MMP-2 concentrations with a limit of detection (LOD) of 0.8 ng/mL. In addition, the developed Cu-PTC MOF also exhibited peroxidase-like activity, which was exploited in the electrochemical detection of MMP-2 subjected to the catalysis of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2. The large surface area of the probe and unique catalytic activity toward TMB facilitated fabrication of an excellent sensing platform to detect MMP-2 with a linear range of 0.5–200 ng/mL with a LOD of 0.25 ng/mL. Various potential interfering biomolecules were evaluated and the stability of the constructed biosensor was assessed in optical and electrochemical modes. The reliability of the electrochemical detection mode was further executed by monitoring MMP-2 in biological samples (human serum and cell samples) and good recoveries were recorded. © 2023 Elsevier B.V.
Original languageBritish English
JournalColloids Surf. A Physicochem. Eng. Asp.
Volume679
DOIs
StatePublished - 2023

Keywords

  • 4
  • 6-tricarboxylate
  • Copper metal organic framework
  • Matrix metalloproteinase-2
  • Nanozyme
  • Opto-electrochemical detection
  • Peptide substrate
  • Peroxidase-like activity
  • Pyridine-2
  • TMB
  • Catalyst activity
  • Copper
  • Crystallinity
  • Electrochemical sensors
  • Fluorescence
  • Metal nanoparticles
  • Metal-Organic Frameworks
  • Morphology
  • Pyridine
  • 3,3,5,5-tetramethylbenzidine
  • Copper metal
  • ELectrochemical detection
  • Metalorganic frameworks (MOFs)
  • Peptide substrates
  • Peroxidase-like activities
  • Tetramethylbenzidine
  • Tipping bucket models
  • Probes
  • carbon dioxide
  • copper
  • gelatinase A
  • glucose oxidase
  • hydrogen peroxide
  • lysozyme
  • metal organic framework
  • myoglobin
  • nanoparticle
  • peroxidase
  • pyridine 2,4,6 tricarboxylate
  • thrombin
  • trypsin
  • unclassified drug
  • absorption
  • Article
  • catalysis
  • controlled study
  • cyclic voltammetry
  • differential pulse voltammetry
  • electrochemical detection
  • environmental temperature
  • enzyme activation
  • enzyme activity
  • fluorescence
  • fluorescence analysis
  • fluorescence intensity
  • human
  • human tissue
  • limit of detection
  • oxidation
  • particle size
  • physisorption
  • protein cleavage
  • scanning electron microscopy
  • spectrofluorometry
  • static electricity
  • substrate concentration
  • thermogravimetry
  • X ray diffraction
  • X ray photoemission spectroscopy
  • zeta potential

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