Continuous flow nanoparticle concentration using alternating current-electroosmotic flow

Kai F. Hoettges, Martin B. Mcdonnell, Michael P. Hughes

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Achieving real-time detection of environmental pathogens such as viruses and bacterial spores requires detectors with both rapid action and a suitable detection threshold. However, most biosensors have detection limits of an order of magnitude or more above the potential infection threshold, limiting their usefulness. This can be improved through the use of automated sample preparation techniques such as preconcentration. In this paper, we describe the use of AC electroosmosis to concentrate nanoparticles from a continuous flow. Electrodes at an optimized angle across a flow cell, and energized by a 1 kHz signal, were used to push nanoparticles to one side of a flow cell, and to extract the resulting stream with a high particle concentration from that side of the flow cell. A simple model of the behavior of particles in the flow cell has been developed, which shows good agreement with experimental results. The method indicates potential for higher concentration factors through cascading devices.

Original languageBritish English
Pages (from-to)467-473
Number of pages7
JournalElectrophoresis
Volume35
Issue number4
DOIs
StatePublished - Feb 2014

Keywords

  • AC-electroosmotic flow
  • Bioparticle detection
  • Dielectrophoresis
  • Lab on a chip
  • Particle separation

Fingerprint

Dive into the research topics of 'Continuous flow nanoparticle concentration using alternating current-electroosmotic flow'. Together they form a unique fingerprint.

Cite this