Chitosan-induced restructuration of a mica-supported phospholipid bilayer: An atomic force microscopy study

Chitosan has emerged as a promising material for biomedical applications. However, the effect of chitosan adsorption on the structure of model biomembrane is not known. In this study, atomic force microscopy (AFM) is employed to investigate the interaction between chitosan and mica-supported dipalmitoylphosphocholine (DPPC) bilayer. First, in situ AFM measurement indicates that nucleation of chitosan occurs around the membrane defects at the initial stage of chitosan incubation. Eventually, DPPC-chitosan binding and chitosan intermolecular association lead to chitosan aggregation on the membrane surface which is quantified by average height measurement and RMS roughness analysis. Lateral force microscopy (LFM) confirms that the adsorbed chitosan has distinct material properties. Furthermore, the trend of surface pressure-area isotherms supports the condensation of DPPC monolayer induced by chitosan in the aqueous subphase. Surface coverage and surface roughness analysis show that the extent of chitosan aggregation on the supported membrane is affected by the incubation time during long-term chitosan incubation.