Characterization of two heterozygous mutations of the oocyte activation factor phospholipase C zeta (PLCζ) from an infertile man by use of minisequencing of individual sperm and expression in somatic cells

Junaid Kashir, Michalis Konstantinidis, Celine Jones, Bjorn Heindryckx, Petra De Sutter, John Parrington, Dagan Wells, Kevin Coward

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Objective: To examine the underlying factors leading to infertility in a male patient from whom phospholipase C zeta H398P (PLCζ H398P, histidine > proline) and PLCζ H233L (histidine > leucine) mutations were previously identified. Design: Laboratory-based study. Setting: University laboratory. Patient(s): An infertile 38-year-old man with significantly impaired oocyte activation ability. Intervention(s): Minisequencing of individual sperm for PLCζ H398P and PLCζ H233L, and investigation of localization patterns arising from the expression of fluorescently tagged PLCζ isoforms in HEK293T cells. Main Outcome Measure(s): The presence/absence of PLCζ H398P and PLCζ H233L determined in individual sperm (n = 12 sperm), and localization of fluorescent mutant PLCζ isoforms quantified in HEK293T cells. Result(s): Sperm possessed either PLCζ H233L or PLCζ H398P, but never both at the same time. Fluorescent PLCζ H233L and PLCζ H233L+H398P (both mutations together) localized to discrete regions in HEK293T cytoplasm but not the plasma membrane. Fluorescence statistically significantly varied between constructs such that PLCζ WT > mutant isoforms at both 48- and 56-hour time points. Fluorescent-PLCζ H233L+H398P exhibited a statistically significantly reduced level of fluorescence compared with PLCζ H398P at 48 hours but not 56 hours. Conclusion(s): Both H398P and H233L mutations are present on different alleles and do not alter PLCζ localization in HEK293T cells. Loss-of-activity mutations in PLCζ may contribute not only toward male infertility but also male subfertility in cases where PLCζ is mutated on a single allele.

Original languageBritish English
Pages (from-to)423-431
Number of pages9
JournalFertility and Sterility
Volume98
Issue number2
DOIs
StatePublished - Aug 2012

Keywords

  • Infertility
  • mutation
  • oocyte activation
  • phospholipase C zeta (PLCzeta)
  • sperm

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