TY - JOUR
T1 - Characterization of optimized TiO2 nanotubes morphology for medical implants
T2 - Biological activity and corrosion resistance
AU - Nogueira, Ricardo Pereira
AU - Uchoa, Jose Deuzimar
AU - Hilario, Fanny
AU - Santana-Melo, Gabriela De Fátima
AU - De Vasconcellos, Luana Marotta Reis
AU - Marciano, Fernanda Roberta
AU - Roche, Virginie
AU - Moreira Jorge Junior, Alberto
AU - Lobo, Anderson Oliveira
N1 - Funding Information:
The authors are grateful to the French Committee for the Evaluation of Academic and Scientific Cooperation with Brazil (COFECUB) and the Brazilian CAPES (Process number #88887.321581/2019-00) for funding the bilateral cooperation and also FAPESP under the grant number for the partial funding FAPESP #2012/13179-6. The authors thank the Laboratory of Electrochemistry and Physico- chemistry of Materials and Interfaces (LEPMI) for using their facilities. AMJJ would like to acknowledge the CNPq (Brazil) under grant #301429/2017-0 and #409847/2016-0. AOL would like to acknowledge the CNPq (Brazil) under grant #310883/2020-2 and #404683/2018-5. FRM would like to acknowledge the CNPq (Brazil) under grant #311531/2020-2.
Funding Information:
The authors are grateful to the French Committee for the Evaluation of Academic and Scientific Cooperation with Brazil (COFECUB) and the Brazilian CAPES (Process number #88887.321581/2019-00) for funding the bilateral cooperation and also F APESP under the grant number for the partial funding F APESP #2012/13179-6. The authors thank the Laboratory of Electrochemistry and Physicochemistry of Materials and Interfaces (LEPMI) for using their facilities. AMJJ would like to acknowledge the CNPq (Brazil) under grant #301429/2017-0 and #409847/2016-0. AOL would like to acknowledge the CNPq (Brazil) under grant #310883/2020-2 and #404683/2018-5. FRM would like to acknowledge the CNPq (Brazil) under grant #31 1531/2020-2.
Publisher Copyright:
© 2021 Nogueira et al.
PY - 2021
Y1 - 2021
N2 - Background: Nanostructured surface modifications of Ti-based biomaterials are moving up from a highly-promising to a successfully-implemented approach to developing safe and reliable implants. Methods: The study’s main objective is to help consolidate the knowledge and identify the more suitable experimental strategies related to TiO2 nanotubes-modified surfaces. In this sense, it proposes the thorough investigation of two optimized nanotubes morphologies in terms of their biological activity (cell cytotoxicity, alkaline phosphatase activity, alizarin red mineralization test, and cellular adhesion) and their electrochemical behavior in simulated body fluid (SBF) electrolyte. Layers of small-short and large-long nanotubes were prepared and investigated in their amorphous and crystallized states and compared to non-anodized samples. Results: Results show that much more than the surface area development associated with the nanotubes’ growth; it is the heat treatment-induced change from amorphous to crystalline anatase-rutile structures that ensure enhanced biological activity coupled to high corrosion resistance. Conclusion: Compared to both non-anodized and amorphous nanotubes layers, the crystal- lized nano-structures’ outstanding bioactivity was related to the remarkable increase in their hydrophilic behavior, while the enhanced electrochemical stability was ascribed to the thickening of the dense rutile barrier layer at the Ti surface beneath the nanotubes.
AB - Background: Nanostructured surface modifications of Ti-based biomaterials are moving up from a highly-promising to a successfully-implemented approach to developing safe and reliable implants. Methods: The study’s main objective is to help consolidate the knowledge and identify the more suitable experimental strategies related to TiO2 nanotubes-modified surfaces. In this sense, it proposes the thorough investigation of two optimized nanotubes morphologies in terms of their biological activity (cell cytotoxicity, alkaline phosphatase activity, alizarin red mineralization test, and cellular adhesion) and their electrochemical behavior in simulated body fluid (SBF) electrolyte. Layers of small-short and large-long nanotubes were prepared and investigated in their amorphous and crystallized states and compared to non-anodized samples. Results: Results show that much more than the surface area development associated with the nanotubes’ growth; it is the heat treatment-induced change from amorphous to crystalline anatase-rutile structures that ensure enhanced biological activity coupled to high corrosion resistance. Conclusion: Compared to both non-anodized and amorphous nanotubes layers, the crystal- lized nano-structures’ outstanding bioactivity was related to the remarkable increase in their hydrophilic behavior, while the enhanced electrochemical stability was ascribed to the thickening of the dense rutile barrier layer at the Ti surface beneath the nanotubes.
KW - Bioactivity
KW - Commercially pure titanium
KW - Surface modification
KW - TiO nanotubes
UR - http://www.scopus.com/inward/record.url?scp=85100346088&partnerID=8YFLogxK
U2 - 10.2147/IJN.S285805
DO - 10.2147/IJN.S285805
M3 - Article
C2 - 33531806
AN - SCOPUS:85100346088
SN - 1176-9114
VL - 16
SP - 667
EP - 682
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
ER -