Abstract
125I-labeled protein D was shown to bind to whole rat epididymal sperm in a time-dependent, saturable, and reversible manner. Scatchard analysis indicates that there are 3.4 x 105 binding sites/sperm cell with a binding efficiency (K(d)) of 2.3 x 10-10 M. Binding of protein D to sperm reaches equilibrium by 95 min. and is reversible by 30 min. Specific binding of protein D to sperm pre-treated with a dilute trypsin solution is reduced significantly when compared with controls. Our findings establish that protein D-specific binding proteins displaying characteristics of a true receptor are present on the surface of rat epididymal sperm.
Original language | British English |
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Pages (from-to) | 1003-1010 |
Number of pages | 8 |
Journal | Biochemistry and Molecular Biology International |
Volume | 40 |
Issue number | 5 |
DOIs | |
State | Published - 1996 |