Cellular expression of α4 subunit mRNA of the nicotinic acetylcholine receptor in the developing rat telencephalon

C. H. Ostermann; J. Grunwald; A. Wevers; D. E. Lorke; S. Reinhardt; A. Maelicke; H. Schröder

doi:10.1016/0304-3940(95)11598-Q

Cellular expression of α4 subunit mRNA of the nicotinic acetylcholine receptor in the developing rat telencephalon

C. H. Ostermann, J. Grunwald, A. Wevers, D. E. Lorke, S. Reinhardt, A. Maelicke, H. Schröder

College of Medicine and Health Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

By contrast to adult brain, little is known on the development of nicotinic acetylcholine receptor (nAChR) expression. Using a digoxigenin-labeled riboprobe for in situ hybridization, α4 nAChR subunit mRNA expression was studied in embryonic and postnatal rat neocortex and hippocampus where it was transiently increased in neuronal subpopulations and preceded cholinergic fiber ingrowth. α4 expression was increased in neocortical layer VIb between E20 and P2 and, about birth, in dentate gyrus granule cells subsequently decreasing to adult levels. nAChR mmRNA expression is increased at the developing neuromuscular endplate preceding cholinergic innervation which triggers changes in non-α nAChR isoform expression. It has to be elucidated whether similar changes may occur in the telencephalon.

Original language	British English
Pages (from-to)	21-24
Number of pages	4
Journal	Neuroscience Letters
Volume	192
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(95)11598-Q
State	Published - 2 Jun 1995

Keywords

Cerebral cortex
Cholinergic transmission
Development
Digoxigenin
Hippocampus
In situ hybridization
Nicotinic acetylcholine receptor
Rat brain

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10.1016/0304-3940(95)11598-Q

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title = "Cellular expression of α4 subunit mRNA of the nicotinic acetylcholine receptor in the developing rat telencephalon",

abstract = "By contrast to adult brain, little is known on the development of nicotinic acetylcholine receptor (nAChR) expression. Using a digoxigenin-labeled riboprobe for in situ hybridization, α4 nAChR subunit mRNA expression was studied in embryonic and postnatal rat neocortex and hippocampus where it was transiently increased in neuronal subpopulations and preceded cholinergic fiber ingrowth. α4 expression was increased in neocortical layer VIb between E20 and P2 and, about birth, in dentate gyrus granule cells subsequently decreasing to adult levels. nAChR mmRNA expression is increased at the developing neuromuscular endplate preceding cholinergic innervation which triggers changes in non-α nAChR isoform expression. It has to be elucidated whether similar changes may occur in the telencephalon.",

keywords = "Cerebral cortex, Cholinergic transmission, Development, Digoxigenin, Hippocampus, In situ hybridization, Nicotinic acetylcholine receptor, Rat brain",

author = "Ostermann, {C. H.} and J. Grunwald and A. Wevers and Lorke, {D. E.} and S. Reinhardt and A. Maelicke and H. Schr{\"o}der",

note = "Funding Information: We are greatly indebted to Prof. S. Heinemann, La Jolla, for providing the clones used for probe construction. We would like to thank Prof. P.G.M. Luiten, Gron-ingen, for valuable comments. We thank A. Jeske, S. Nowacki and K. Pilz for excellent technical assistance, I. Koch for photographical and A. Kirchmayer for secretarial work. This study was supported by the Deutsche For-schungsgemeinschaft (Schr 283/8-2).",

year = "1995",

month = jun,

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doi = "10.1016/0304-3940(95)11598-Q",

language = "British English",

volume = "192",

pages = "21--24",

journal = "Neuroscience Letters",

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T1 - Cellular expression of α4 subunit mRNA of the nicotinic acetylcholine receptor in the developing rat telencephalon

AU - Ostermann, C. H.

AU - Grunwald, J.

AU - Wevers, A.

AU - Lorke, D. E.

AU - Reinhardt, S.

AU - Maelicke, A.

AU - Schröder, H.

N1 - Funding Information: We are greatly indebted to Prof. S. Heinemann, La Jolla, for providing the clones used for probe construction. We would like to thank Prof. P.G.M. Luiten, Gron-ingen, for valuable comments. We thank A. Jeske, S. Nowacki and K. Pilz for excellent technical assistance, I. Koch for photographical and A. Kirchmayer for secretarial work. This study was supported by the Deutsche For-schungsgemeinschaft (Schr 283/8-2).

PY - 1995/6/2

Y1 - 1995/6/2

N2 - By contrast to adult brain, little is known on the development of nicotinic acetylcholine receptor (nAChR) expression. Using a digoxigenin-labeled riboprobe for in situ hybridization, α4 nAChR subunit mRNA expression was studied in embryonic and postnatal rat neocortex and hippocampus where it was transiently increased in neuronal subpopulations and preceded cholinergic fiber ingrowth. α4 expression was increased in neocortical layer VIb between E20 and P2 and, about birth, in dentate gyrus granule cells subsequently decreasing to adult levels. nAChR mmRNA expression is increased at the developing neuromuscular endplate preceding cholinergic innervation which triggers changes in non-α nAChR isoform expression. It has to be elucidated whether similar changes may occur in the telencephalon.

AB - By contrast to adult brain, little is known on the development of nicotinic acetylcholine receptor (nAChR) expression. Using a digoxigenin-labeled riboprobe for in situ hybridization, α4 nAChR subunit mRNA expression was studied in embryonic and postnatal rat neocortex and hippocampus where it was transiently increased in neuronal subpopulations and preceded cholinergic fiber ingrowth. α4 expression was increased in neocortical layer VIb between E20 and P2 and, about birth, in dentate gyrus granule cells subsequently decreasing to adult levels. nAChR mmRNA expression is increased at the developing neuromuscular endplate preceding cholinergic innervation which triggers changes in non-α nAChR isoform expression. It has to be elucidated whether similar changes may occur in the telencephalon.

KW - Cerebral cortex

KW - Cholinergic transmission

KW - Development

KW - Digoxigenin

KW - Hippocampus

KW - In situ hybridization

KW - Nicotinic acetylcholine receptor

KW - Rat brain

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DO - 10.1016/0304-3940(95)11598-Q

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AN - SCOPUS:0028988515

SN - 0304-3940

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EP - 24

JO - Neuroscience Letters

JF - Neuroscience Letters

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ER -

Cellular expression of α4 subunit mRNA of the nicotinic acetylcholine receptor in the developing rat telencephalon

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