Bypassing the proline/thiazoline requirement of the macrocyclase PatG

E. Oueis, H. Stevenson, M. Jaspars, N. J. Westwood, J. H. Naismith

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Biocatalysis is a fast developing field in which an enzyme's natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified two synthetic routes, triazole and a double cysteine, that circumvent this requirement. With the triazole, we show PatGmac can macrocyclise substrates that do not contain any amino acids in the final product.

Original languageBritish English
Pages (from-to)12274-12277
Number of pages4
JournalChemical Communications
Issue number91
StatePublished - 2017


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