TY - JOUR
T1 - Bromelain
T2 - An overview of industrial application and purification strategies
AU - Arshad, Zatul Iffah Mohd
AU - Amid, Azura
AU - Yusof, Faridah
AU - Jaswir, Irwandi
AU - Ahmad, Kausar
AU - Loke, Show Pau
PY - 2014/9
Y1 - 2014/9
N2 - This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.
AB - This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.
KW - Bromelain
KW - Cysteine protease
KW - Purification
KW - Recombinant bromelain
UR - http://www.scopus.com/inward/record.url?scp=84906948417&partnerID=8YFLogxK
U2 - 10.1007/s00253-014-5889-y
DO - 10.1007/s00253-014-5889-y
M3 - Short survey
C2 - 24965557
AN - SCOPUS:84906948417
SN - 0175-7598
VL - 98
SP - 7283
EP - 7297
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 17
ER -