Bromelain: An overview of industrial application and purification strategies

Zatul Iffah Mohd Arshad, Azura Amid, Faridah Yusof, Irwandi Jaswir, Kausar Ahmad, Show Pau Loke

Research output: Contribution to journalShort surveypeer-review

160 Scopus citations


This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.

Original languageBritish English
Pages (from-to)7283-7297
Number of pages15
JournalApplied Microbiology and Biotechnology
Issue number17
StatePublished - Sep 2014


  • Bromelain
  • Cysteine protease
  • Purification
  • Recombinant bromelain


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