Attenuation of Endoplasmic Reticulum Stress, Impaired Calcium Homeostasis, and Altered Bioenergetic Functions in MPP+-Exposed SH-SY5Y Cells Pretreated with Rutin

Adaze Bijou Enogieru, William Lloyd Haylett, Hayley Christy Miller, Francois Hendrikus van der Westhuizen, Donavon Charles Hiss, Okobi Eko Ekpo

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Parkinson’s disease (PD) is a common neurodegenerative disorder that affects approximately 1% of the population over the age of 65 years. While treatment options for PD are limited, reports show that plant-derived bioactive compounds such as rutin possess numerous pharmacological benefits, including antioxidant and antiapoptotic activities. This study aimed to investigate the potential role of rutin in MPP+-treated SH-SY5Y neuroblastoma cells, an established cell model of PD. Our findings reveal increased concentrations of Ca2+ and endoplasmic reticulum (ER) stress as well as impaired mitochondrial membrane potential and bioenergetic status in SH-SY5Y cells treated with MPP+ only. This is demonstrated by a significant reduction in the expression levels of BiP, significantly reduced basal respiration, maximal respiration, and spare respiratory capacity as well as a significant increase in the expression levels of CHOP; however, these effects were significantly attenuated following pretreatment with rutin. Also, rutin significantly improved basal and compensatory glycolysis as a response to an impaired oxidative phosphorylation system triggered by MPP+, characterized by deficient ATP production. In conclusion, our findings provide the first evidence on the ability of rutin to maintain Ca2+ homeostasis, inhibit ER stress, and protect the mitochondria in MPP+-treated SH-SY5Y cells.

Original languageBritish English
Pages (from-to)764-776
Number of pages13
JournalNeurotoxicity Research
Volume36
Issue number4
DOIs
StatePublished - 1 Nov 2019

Keywords

  • ER stress
  • Glycolysis
  • Oxidative phosphorylation
  • Parkinson’s disease
  • Rutin

Fingerprint

Dive into the research topics of 'Attenuation of Endoplasmic Reticulum Stress, Impaired Calcium Homeostasis, and Altered Bioenergetic Functions in MPP+-Exposed SH-SY5Y Cells Pretreated with Rutin'. Together they form a unique fingerprint.

Cite this