Assessment of RNALater ® as a potential method to preserve bovine muscle proteins compared with dry ice in a proteomic study

Yao Zhu, Anne Maria Mullen, Dilip K. Rai, Alan L. Kelly, David Sheehan, Jamie Cafferky, Ruth M. Hamill

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

RNAlater ® is regarded as a potential preservation method for proteins, while its effect on bovine muscle proteins has rarely been evaluated. Bovine muscle protein samples (n = 12) collected from three tender (Warner-Bratzler shear force: 30.02-31.74 N) and three tough (Warner-Bratzler shear force: 54.12-66.25 N) Longissimus thoracis et lumborum (LTL) samples, preserved using two different sampling preservation methods (RNAlater ® and dry ice), at two post mortem time points (day 0 and day 14), were characterized using one-dimensional electrophoresis. Fourteen bands with molecular weights ranging from 15 to 250 kDa were verified, both in the dry ice and RNAlater ® storage groups, at each time point, using image analysis. A shift from high to low molecular weight fragments, between day 0 and day 14, indicated proteolysis of the muscle proteins during post mortem storage. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses and database searching resulted in the identification of 10 proteins in four bands. Protein profiles of muscle preserved in RNAlater ® were similar to those of muscle frozen on dry ice storage, both at day 0 and day 14. The results demonstrate that RNAlater ® could be a simple and efficient way to preserve bovine muscle proteins for bovine muscle proteomic studies.

Original languageBritish English
Article number60
JournalFoods
Volume8
Issue number2
DOIs
StatePublished - Feb 2019

Keywords

  • Bovine proteomics
  • LC-MS/MS
  • Muscle proteins
  • One-dimensional electrophoresis
  • Sample preparation

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