TY - JOUR
T1 - Application of thermo-separating aqueous two-phase system in extractive bioconversion of polyhydroxyalkanoates by Cupriavidus necator H16
AU - Leong, Yoong Kit
AU - Show, Pau Loke
AU - Lan, John Chi Wei
AU - Krishnamoorthy, Rambabu
AU - Chu, Dinh Toi
AU - Nagarajan, Dillirani
AU - Yen, Hong Wei
AU - Chang, Jo Shu
N1 - Funding Information:
This study is supported by the Fundamental Research Grant Scheme (Malaysia, FRGS/1/2015/SG05/UNIM/03/1 ; FP005-2013B ), the Ministry of Science and Technology , ( MOSTI 02-02-12-SF0256 ), and the Prototype Research Grant Scheme (Malaysia, PRGS/2/2015/SG05/UNIM/03/1 ). The authors also appreciate the financial support of DDS Institute of Advanced Education, Taiwan .
Funding Information:
This study is supported by the Fundamental Research Grant Scheme (Malaysia, FRGS/1/2015/SG05/UNIM/03/1; FP005-2013B), the Ministry of Science and Technology, (MOSTI 02-02-12-SF0256), and the Prototype Research Grant Scheme (Malaysia, PRGS/2/2015/SG05/UNIM/03/1). The authors also appreciate the financial support of DDS Institute of Advanced Education, Taiwan.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/9
Y1 - 2019/9
N2 - Polyhydroxyalkanoates (PHAs), a family of biodegradable and renewable biopolymers show a huge potential as an alternative to conventional plastics. Extractive bioconversion (in situ product recovery)is a technique that integrates upstream fermentation and downstream purification. In this study, extractive bioconversion of PHAs from Cupriavidus necator H16 was performed via a thermo-separating aqueous two-phase system to reduce the cost and environmental impacts of PHAs production. Key operating parameters, such as polymer concentration, temperature, and pH, were optimized. The strategy achieved a yield and PF of 97.6% and 1.36-fold, respectively at 5% EOPO 3900 concentration, 30 °C fermentation temperature and pH 6. The PHAs production process was also successfully scaled up in a 2 L bioreactor. To the best of our knowledge, this is the first report on extractive fermentation of PHAs from Cupriavidus necator utilizing a thermo-separation system to achieve a better productivity and purity of the target product.
AB - Polyhydroxyalkanoates (PHAs), a family of biodegradable and renewable biopolymers show a huge potential as an alternative to conventional plastics. Extractive bioconversion (in situ product recovery)is a technique that integrates upstream fermentation and downstream purification. In this study, extractive bioconversion of PHAs from Cupriavidus necator H16 was performed via a thermo-separating aqueous two-phase system to reduce the cost and environmental impacts of PHAs production. Key operating parameters, such as polymer concentration, temperature, and pH, were optimized. The strategy achieved a yield and PF of 97.6% and 1.36-fold, respectively at 5% EOPO 3900 concentration, 30 °C fermentation temperature and pH 6. The PHAs production process was also successfully scaled up in a 2 L bioreactor. To the best of our knowledge, this is the first report on extractive fermentation of PHAs from Cupriavidus necator utilizing a thermo-separation system to achieve a better productivity and purity of the target product.
KW - Aqueous two-phase extraction
KW - Extractive bioconversion
KW - PHB purification
KW - Polyhydroxyalkanoates
KW - Thermo-separating polymer
UR - https://www.scopus.com/pages/publications/85065790301
U2 - 10.1016/j.biortech.2019.121474
DO - 10.1016/j.biortech.2019.121474
M3 - Article
C2 - 31122870
AN - SCOPUS:85065790301
SN - 0960-8524
VL - 287
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 121474
ER -