TY - JOUR
T1 - Application of statistical modeling for the production of highly pure rhamnolipids using magnetic biocatalysts
T2 - Evaluating its efficiency as a bioremediation agent
AU - Rathankumar, Abiram Karanam
AU - Saikia, Kongkona
AU - Ribeiro, Maria H.
AU - Cheng, Chin Kui
AU - Purushothaman, Maheswari
AU - Vaidyanathan, Vinoth kumar
N1 - Funding Information:
Vinoth Kumar Vaidyanathan expresses his deepest gratitude to the support extended by the All India Council for Technical Education − Research Promotion Scheme (8–60/FDC/RPS (POLICY-1)/2019–20) and Department of Science and Technology, India (DST/INT/MEXICO/P-13/2016).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/6/15
Y1 - 2021/6/15
N2 - In the present study, highly pure rhamnolipids (RLs) was produced using biocatalysts immobilized on amino-functionalized chitosan coated magnetic nanoparticles. Upon immobilizing naringinase and Candida antarctica lipase B (CaLB) under the optimized conditions, an enhanced operational stability with biocatalytic loads of 935 ± 2.4 U/g (naringinase) and 825 ± 4.1 U/g (CaLB) were achieved. Subsequently, the immobilized biocatalysts were utilized sequentially in a two-step RLs synthesis process. The key parameters involved in RLs production were optimized using artificial neural network (ANN) coupled genetic algorithm (GA) and were compared with composite central design (CCD). On validating the efficiency of both models, mean square errors of 1.58% (CCD) and 1.04% (ANN) were obtained. Optimization of parameters by ANN-GA resulted in 1.2-fold increase in experimental RLs yield (80.53%), which was 1.05-fold higher when compared to CCD model. Further, to establish the efficiency of RLs as a bioremediation agent, it was utilized as washing agent. It was observed that at a soil to RLs volume of 1:05, RLs concentration of 0.4 mg/mL, a 95.35 ± 1.33% removal of Total Petroleum Hydrocarbons (TPHs) was obtained at 35 ℃ and 160 rpm in 75 min. Thus, this strategy provides an efficient biocatalytic toolbox for RLs synthesis, which can be effectively used as a bioremediation agent.
AB - In the present study, highly pure rhamnolipids (RLs) was produced using biocatalysts immobilized on amino-functionalized chitosan coated magnetic nanoparticles. Upon immobilizing naringinase and Candida antarctica lipase B (CaLB) under the optimized conditions, an enhanced operational stability with biocatalytic loads of 935 ± 2.4 U/g (naringinase) and 825 ± 4.1 U/g (CaLB) were achieved. Subsequently, the immobilized biocatalysts were utilized sequentially in a two-step RLs synthesis process. The key parameters involved in RLs production were optimized using artificial neural network (ANN) coupled genetic algorithm (GA) and were compared with composite central design (CCD). On validating the efficiency of both models, mean square errors of 1.58% (CCD) and 1.04% (ANN) were obtained. Optimization of parameters by ANN-GA resulted in 1.2-fold increase in experimental RLs yield (80.53%), which was 1.05-fold higher when compared to CCD model. Further, to establish the efficiency of RLs as a bioremediation agent, it was utilized as washing agent. It was observed that at a soil to RLs volume of 1:05, RLs concentration of 0.4 mg/mL, a 95.35 ± 1.33% removal of Total Petroleum Hydrocarbons (TPHs) was obtained at 35 ℃ and 160 rpm in 75 min. Thus, this strategy provides an efficient biocatalytic toolbox for RLs synthesis, which can be effectively used as a bioremediation agent.
KW - Biocatalysts
KW - Immobilization
KW - Modeling
KW - Rhamnolipids
KW - Soil washing
UR - http://www.scopus.com/inward/record.url?scp=85100693143&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2021.125323
DO - 10.1016/j.jhazmat.2021.125323
M3 - Article
C2 - 33951876
AN - SCOPUS:85100693143
SN - 0304-3894
VL - 412
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 125323
ER -
