Analysis of pralidoxime in serum, brain and CSF of rats

Huba Kalász; Éva Szöko; Tamás Tábi; Georg Petroianu; Dietrich E. Lorke; Abdulrab Omar; Salem Alafifi; Almerri Jasem; Kornélia Tekes

doi:10.2174/157340609788185882

Analysis of pralidoxime in serum, brain and CSF of rats

Huba Kalász
, Éva Szöko
, Tamás Tábi
, Georg Petroianu
, Dietrich E. Lorke
, Abdulrab Omar
, Salem Alafifi
, Almerri Jasem
, Kornélia Tekes

College of Medicine and Health Sciences

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 - 200 μg/mL, 0.3 - 7 μg/mL and 0.1 - 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoxime-type antidotes clinically used in organophosphate poisoning.

Original language	British English
Pages (from-to)	237-241
Number of pages	5
Journal	Medicinal Chemistry
Volume	5
Issue number	3
DOIs	https://doi.org/10.2174/157340609788185882
State	Published - 2009

Keywords

Brain
Cerebrospinal fluid (CSF)
CZE
Pralidoxime
Serum

Access to Document

10.2174/157340609788185882

OpenUrl availability

Full text

Cite this

@article{bb104fa5dced4249a397fcb6886c9c54,

title = "Analysis of pralidoxime in serum, brain and CSF of rats",

abstract = "After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 - 200 μg/mL, 0.3 - 7 μg/mL and 0.1 - 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoxime-type antidotes clinically used in organophosphate poisoning.",

keywords = "Brain, Cerebrospinal fluid (CSF), CZE, Pralidoxime, Serum",

author = "Huba Kal{\'a}sz and {\'E}va Sz{\"o}ko and Tam{\'a}s T{\'a}bi and Georg Petroianu and Lorke, \{Dietrich E.\} and Abdulrab Omar and Salem Alafifi and Almerri Jasem and Korn{\'e}lia Tekes",

year = "2009",

doi = "10.2174/157340609788185882",

language = "British English",

volume = "5",

pages = "237--241",

journal = "Medicinal Chemistry",

issn = "1573-4064",

publisher = "Bentham Science Publishers",

number = "3",

}

TY - JOUR

T1 - Analysis of pralidoxime in serum, brain and CSF of rats

AU - Kalász, Huba

AU - Szöko, Éva

AU - Tábi, Tamás

AU - Petroianu, Georg

AU - Lorke, Dietrich E.

AU - Omar, Abdulrab

AU - Alafifi, Salem

AU - Jasem, Almerri

AU - Tekes, Kornélia

PY - 2009

Y1 - 2009

N2 - After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 - 200 μg/mL, 0.3 - 7 μg/mL and 0.1 - 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoxime-type antidotes clinically used in organophosphate poisoning.

AB - After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 - 200 μg/mL, 0.3 - 7 μg/mL and 0.1 - 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoxime-type antidotes clinically used in organophosphate poisoning.

KW - Brain

KW - Cerebrospinal fluid (CSF)

KW - CZE

KW - Pralidoxime

KW - Serum

UR - https://www.scopus.com/pages/publications/67651174580

U2 - 10.2174/157340609788185882

DO - 10.2174/157340609788185882

M3 - Article

C2 - 19442213

AN - SCOPUS:67651174580

SN - 1573-4064

VL - 5

SP - 237

EP - 241

JO - Medicinal Chemistry

JF - Medicinal Chemistry

IS - 3

ER -

Analysis of pralidoxime in serum, brain and CSF of rats

Abstract

Keywords

Access to Document

OpenUrl availability

Other files and links

Fingerprint

Cite this